restriction endoluceases Flashcards
1
Q
names of enzymes
A
restriction endonuclease
restriction enzymes
2
Q
what do RE do
A
there are many RE
each one recognises a specific palindromic sequence
3
Q
stages of restriction enzyme
A
enzyme cut a DNA molecule at the specific palindromic sequence - recognition sequence
breaks phosphodiester bonds between adjacent nucleotides
generates sticky ends
4
Q
sticky ends
A
unpaired bases that can form H-bonds with complimentary bases