comparison

Question 1

template

Answer 1

gene machine - sequence programmed into computer

restriction enzymes - DNA

reverse transcriptase - mRNA

Question 2

tissue specificity

Answer 2

gene machine - sequence obtained from human genome project ect

restriction enzyme - any cell

reverse transcriptase - must be from cell in which that gene is transcribed

Question 3

enzyme used

Answer 3

gene machine -

restriction enzyme - restriction enzye

reverse transcriptase - reverse transcriptase

Question 4

introns

Answer 4

gene machine - choice of programming in sequence with or without introns

restriction enzyme - yes as DNA from nucleus is used

reverse transcriptase - no as mRnA has already had introns removed by splicing

Question 5

product formed

Answer 5

gene machine - gene

restriction enzyme - DNA/gene obtained is identical to the gene in cells

reverse transcriptase - cDNA lacks introns