Respiratory Chain Flashcards
NAD means…
Nicotinamide adenine dinucleotide
FAD means…
Flavin adenine dinucleotide
Name a complex I inhibitor.
Rotenone
Name a complex II inhibitor.
Malonate
Name a complex III inhibitor.
Antimycin A
Name a complex IV inhibitor.
Cyanide
How do you measure H+ pumping and e- transport?
using pH electrodes
- measure pH change after O2 added as H+ goes back through ATP synthase
- only starts changing once O2 added as final e- accepter
Stoichiometry
determining the amount of products/reactants that are produced/needed in a given reaction
What’s the significance of calculating stoichiometries?
can propose & test models of mechanisms of H+ pumping
UQH2
Ubiquinol
UQ
Ubiquinone
List the redox centres in complex I?
~ NAD binding site
~ 7 Fe-S centres
~ FMN
~ tightly bound UQ
How many subunits are used in complex I?
41
~ 7 integral to the membrane
~ 34 peripheral
(in the mitochondria) CHECK
What electron donor is used in complex II?
Succinate
How many subunits are used in complex III?
3 Fe containing subunits
List the redox centres in complex III.
~ Rieske protein - 2Fe/S centres
~ cytochrome C1 - haem group
~ cytochrome B - two ahem groups = BL + BH
Haem groups
iron containing redox centres
~ they have diff affinities depending on their position
For every 2e- pumped through complex I, II + III how many H+ are also pumped?
Complex I = 4
Complex II = 0 as no H+ pumping
Complex III = 4
What electron accepter is used in complex IV?
final electron accepter = O2
List the transmembrane spanning domains and the redox centres for subunits I + II for complex IV.
I = 12 + haem, a, a3, CuB II = 2 + CuA
List the transmembrane spanning domains and the redox centres for subunits III + IV for complex IV.
III = 7 + none IV = 1 + none
