Respiratory Chain Flashcards

1
Q

NAD means…

A

Nicotinamide adenine dinucleotide

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2
Q

FAD means…

A

Flavin adenine dinucleotide

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3
Q

Name a complex I inhibitor.

A

Rotenone

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4
Q

Name a complex II inhibitor.

A

Malonate

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5
Q

Name a complex III inhibitor.

A

Antimycin A

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6
Q

Name a complex IV inhibitor.

A

Cyanide

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7
Q

How do you measure H+ pumping and e- transport?

A

using pH electrodes

  • measure pH change after O2 added as H+ goes back through ATP synthase
  • only starts changing once O2 added as final e- accepter
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8
Q

Stoichiometry

A

determining the amount of products/reactants that are produced/needed in a given reaction

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9
Q

What’s the significance of calculating stoichiometries?

A

can propose & test models of mechanisms of H+ pumping

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10
Q

UQH2

A

Ubiquinol

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11
Q

UQ

A

Ubiquinone

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12
Q

List the redox centres in complex I?

A

~ NAD binding site
~ 7 Fe-S centres
~ FMN
~ tightly bound UQ

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13
Q

How many subunits are used in complex I?

A

41
~ 7 integral to the membrane
~ 34 peripheral
(in the mitochondria) CHECK

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14
Q

What electron donor is used in complex II?

A

Succinate

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15
Q

How many subunits are used in complex III?

A

3 Fe containing subunits

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16
Q

List the redox centres in complex III.

A

~ Rieske protein - 2Fe/S centres
~ cytochrome C1 - haem group
~ cytochrome B - two ahem groups = BL + BH

17
Q

Haem groups

A

iron containing redox centres

~ they have diff affinities depending on their position

18
Q

For every 2e- pumped through complex I, II + III how many H+ are also pumped?

A

Complex I = 4
Complex II = 0 as no H+ pumping
Complex III = 4

19
Q

What electron accepter is used in complex IV?

A

final electron accepter = O2

20
Q

List the transmembrane spanning domains and the redox centres for subunits I + II for complex IV.

A
I = 12 + haem, a, a3, CuB
II = 2 + CuA
21
Q

List the transmembrane spanning domains and the redox centres for subunits III + IV for complex IV.

A
III = 7 + none
IV = 1 + none