Respiratory Chain Flashcards
NAD means…
Nicotinamide adenine dinucleotide
FAD means…
Flavin adenine dinucleotide
Name a complex I inhibitor.
Rotenone
Name a complex II inhibitor.
Malonate
Name a complex III inhibitor.
Antimycin A
Name a complex IV inhibitor.
Cyanide
How do you measure H+ pumping and e- transport?
using pH electrodes
- measure pH change after O2 added as H+ goes back through ATP synthase
- only starts changing once O2 added as final e- accepter
Stoichiometry
determining the amount of products/reactants that are produced/needed in a given reaction
What’s the significance of calculating stoichiometries?
can propose & test models of mechanisms of H+ pumping
UQH2
Ubiquinol
UQ
Ubiquinone
List the redox centres in complex I?
~ NAD binding site
~ 7 Fe-S centres
~ FMN
~ tightly bound UQ
How many subunits are used in complex I?
41
~ 7 integral to the membrane
~ 34 peripheral
(in the mitochondria) CHECK
What electron donor is used in complex II?
Succinate
How many subunits are used in complex III?
3 Fe containing subunits