Respiration Exam Ques Flashcards
Glycolysis
- phosphorylation of glucose to glucose phosphate from the hydrolysis of 2 x ATP
- splitting of glucose phosphate into 2x 3 carbon triose phosphate
- triose phosphate is oxidised to pyruvate ( Hydrogen is remove and transferred to NAD to form NADH)
+ 2 X atp are regenerated from adp
Why is glucose phosphorylated
To be made more reactive
The hydrolysis of atp provides energy to activate glucose and lowers the activation energy for enzyme controlled reactions
Link reaction
- Pyruvate molecules are oxidised to acetate - loses one co2 and two H: the H atoms are accepted by NAD to form NADH which is used later to produce ATP
- two carbon acetate combines with a molecule called co-enzyme A to produce acetyl co enzyme A
Krebs’ cycle
- acetyl coenzyme A combines with a 4C molecule = 6C molecule ( coenzyme A recycled)
- in a series of redox reactions , 6C molecules loses CO2 and hydrogen to form a 4C molecule and a single ATP
- 4C molecule is able to combine with a new acetyl coenzyme a
How is ATP produced in the Krebs’ cycle
Product of SUBSTRATE LEVEL PHOSPHORYLATION which is the creation of atp without atp synthase - a phosphate is added to adp from another molecule
Oxidative phosphorylation
- NADH + FADH from glycolysis and krebs’ cycle donate electrons of the h atoms to the first molecules in the ETC.
- the electrons pass along a chain of electron transfer carrier molecules in a series of redox reactions
- energy is released which causes active transport of protons across the membrane through atp synthase channels
- for every proton, a molecule of ATP is synthesised
- at the end of the chain the electrons combine with protons and oxygen to form water
Explain why oxygen is needed for the production of ATP on the Cristae of the mitochondrion
Oxygen accepts the electrons in the electron transport chain where atp is formed
Suggest the advantages of the change in blood flow in blood vessels during exercise (3 marks)
- more blood flow to muscles during exercise
- supplying more glucose and oxygen
- for high rate of respiration
Aerobic respiration produces more atp per molecule of glucose than aerobic respiration. Explain why (2 marks)
Oxygen is the terminal electron acceptor in oxidative phosphorylation that only occurs in aerobic respiration
Anaerobic respiration only glycolysis occurs
Suggest why having a large number of mitochondria with this unusual ultrastructure could lead to the neurone dying
- fewer Cristae
- so less oxidative phosphorylation
- so not enough atp produced to keep neurones alive
For the first 10 minutes, the tap attached to the tube a was left open and the syringe from tube b was removed. Suggest three reasons why the apparatus was left for 10 minutes ( 3marks)
- equilibrium reached
- allow for pressure change
- allow the respiration rate of the seeds to stabilise
Suggest and explain why the chosen temperature was 20C for this experiment (2marks)
- optimum temperature for normal growth of seeds
- optimum temperature for enzymes involved in respirarion
During the experiment, the coloured liquid in the tubing moved towards tube b. Explain what caused this (3 marks)
- oxygen taken up/ used by seeds
- CO2 given out is absorbed by KOH
- so pressure in B decreases
Mitochondrial disease often causes muscle weakness. Use your knowledge of respiration and muscle contraction to suggest explanations for this effect of MD ( 3 marks )
- reduction in ATP production by aerobic respiration
- less force generated because fewer actin and myosin interactions in muscle
- fatigue caused by lactate from anaerobic respiration
If someone’s has MD, the concentration of lactate in their blood after exercise is usually much higher than normal. Suggest why ( 3 marks )
- mitochondria not producing much ATP
- with MD increased use of atp supplied by increase in anaerobic respiration
- more lactate produced and leaves muscles by difusión