respiration Flashcards

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1
Q

What is the net yield of ATP produced in glycolysis?

A

The net yield of ATP produced in glycolysis is 2 ATP.

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2
Q

How is ATP produced in the Krebs cycle?

A

One ATP is produced by substrate-level phosphorylation per turn of the Krebs cycle when a 5-carbon compound is converted into oxaloacetate.

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3
Q

Where does chemiosmosis occur?

A

Chemiosmosis occurs in the mitochondria, specifically on the inner membrane and matrix.

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4
Q

What is the role of ATP synthase in chemiosmosis?

A

ATP synthase is a channel protein that facilitates the movement of hydrogen ions across the membrane to produce ATP from ADP and Pi.

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5
Q

What happens to hydrogen ions during chemiosmosis?

A

Hydrogen ions are pumped out of the matrix into the intermembrane space, creating a proton gradient.
- via proton - motive force H+ ions pass through hydrophilic transmembrane protien cristae

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6
Q

What is the relationship between photosynthesis and respiration regarding ATP?

A

Both photosynthesis and respiration make ATP using proton gradients.

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7
Q

How does storing fruit at low temperatures affect respiration?

A

Storing fruit at low temperatures stops respiration.

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8
Q

What is the effect of congenital hypothyroidism on respiration?

A

In congenital hypothyroidism, there are less thyroid hormones, leading to less aerobic respiration, so less ATP produced , so less metabloism + lower body temp

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9
Q

What are the products of glycolysis?

A

Glycolysis produces a total of 4 ATP, with a net production of 2 ATP, and reduced NAD, + 2 pyruvate

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10
Q

What happens to pyruvate in the presence of cyanide?

A

When cyanide is present, pyruvate does not enter the mitochondria, and some carbon dioxide is produced when pyruvate is converted to ethanal via anaerobic respiration.

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11
Q

What is the overall equation for respiration?

A
  • The overall equation for respiration is an oversimplification as glucose is not the only substrate; other substrates like fatty acids and glycerol are also used.
  • respiratim is not a single step , so there are other steps involved + toher products
  • enzymes are involved in resperation processes such as dehydrogenation , decarboylation
  • coenzymes involved
  • glucose doesnt directly react w/ oxygen , instead O2 is used in oxidative phosphorylation as the final e- acceptor
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12
Q

What is produced during the link reaction and Krebs cycle?

A

Carbon dioxide is produced during the link reaction and Krebs cycle, and oxygen is used in oxidative phosphorylation.

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13
Q

Can anaerobic respiration occur in the presence of cyanide?

A

Yes, anaerobic respiration can still occur in the presence of cyanide, as pyruvate is converted to ethanal in the cytoplasm.
then ethanal–> ethanol + this doesnt invovle cytochromes . the e-transport chain , krebs cycle

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14
Q

What does oxidative phosphorylation produce?

A

Oxidative phosphorylation produces ATP, water, and oxidised NAD and FAD.

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15
Q

What is a respiratory substrate?

A

A respiratory substrate is a biological molecule that can be broken down in respiration to release energy.

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16
Q

What is the respiratory quotient of a carbohydrate?

A

The respiratory quotient of a carbohydrate is typically 1.0.

17
Q

How can a respirometer be used?

A
  • used to measure oxygen uptake of living organisms.
  • Potassium hydroxide or soda lime can be used to absorb the CO2 produced
  • you would use a syringe to set the manometer fluid level
  • leave for suitable length of time minimum 20 minutes before measuring the distance moved by the fluid.
  • You would then calculate volume of oxygen taken up per minute, then do repeats and calculate a mean.
  • You could also to set up a of control tube (containing the same mass of beads as there is mass of organism in the test condition, so that there is not more gas in the control tube than the experimental tube). -You would calculate volume of oxygen used by multiplying distance moved by fluid in capillary by 2πr
18
Q

Where do glycolysis and the Krebs cycle take place?

A

Glycolysis takes place in the cytoplasm, while the Krebs cycle takes place in the matrix of the mitochondrion.

19
Q

What happens to pyruvate during anaerobic respiration in animals?

A
  • pyruvate is converted to lactate
  • pyruvate accepts hydrogen atoms from reduced NAD
  • reaction catalases by lactate dehydrogenase
  • as there is no O2 to act as final acceptor - krebs, link reactions cannot take place
  • NAD able to be reused, glycolysis continue + small amount of ATP produced
20
Q

How are animals adapted to respire underwater?

A

Animals respiring underwater have adaptations like large open nostrils, high vital capacity lungs, low metabolic rates, and the ability to respire anaerobically.

21
Q

What is the role of ATP?

A

The role of ATP is to transfer energy/ releases energy.
It is the universal energy molecule/energy intermediate and is an immediate source of energy as its phosphates can be removed by hydrolysis to release 30 kJ mol-1 of energy. The energy is released for metabolism, and ADP can attach a phosphate (forming ATP) during respiration and photosynthesis. ATP releases energy in small amounts to prevent cell damage

22
Q

How does the substrate respired change in hibernating animals?

A

Initially, hibernating animals respire mostly glucose, and over time, they respire more lipid as glucose is used up.

23
Q

What is chronic fatigue syndrome related to in terms of respiration?

A

Chronic fatigue syndrome results in less pyruvate for the link reaction and Krebs cycle, leading to reduced ATP production and muscle weakness.

24
Q

What is the final product of anaerobic respiration in animals?

A

The final product of anaerobic respiration in animals is lactate.

25
Q

What is the final product of anaerobic respiration in yeast?

A

In yeast, the final product of anaerobic respiration is ethanol, with carbon dioxide produced.

26
Q

What is the effect of soaking peas on their respiratory rate?

A

Soaked peas have a higher respiratory rate than dry peas due to the need for an aqueous medium for enzyme and substrate movement.

27
Q

What happens to respiration at higher temperatures?

A

At higher temperatures, respiration occurs faster due to increased kinetic energy of enzymes and substrates.

28
Q

How does temperature affect respiration rate?

A

The respiration rate doubles with a 10°C temperature increase.

29
Q

What are the best storage conditions for fruits and vegetables?

A

Fruits and vegetables are best stored at lower temperatures to keep respiration rates low and reduce spoilage.

30
Q

What adaptation do parasites have for respiration?

A

Parasites living in the blood of mammals respire anaerobically due to limited access to dissolved oxygen.

31
Q

Can anaerobic respiration in mammals be reversed?

A

Yes, anaerobic respiration in mammals can be reversed as pyruvate is converted into lactate.

32
Q

Why does respiration yield fewer ATP molecules than its theoretical maximum?

A

Respiration yields fewer ATP molecules due to energy losses in transport and inefficiencies in the electron transport chain.

33
Q

How does anaerobic respiration compare to aerobic respiration in terms of ATP production?

A

Anaerobic respiration produces less ATP than aerobic respiration, as only substrate-level phosphorylation occurs.

34
Q

How are ATP and DNA nucleotides similar and different?

A

ATP and DNA nucleotides both have a pentose, phosphate, and nitrogenous base, but differ in sugar type and number of phosphates.

35
Q

How is ATP produced in respiration?

A

ATP is produced in respiration by substrate-level phosphorylation and by chemiosmosis/oxidative phosphorylation.

36
Q

How are proteins respired?

A

Proteins are hydrolysed by protease enzymes, deaminated, and enter the Krebs cycle.