Respiration Flashcards
mitochondria components
- outer membrane
- inner membrane
- cristae
- matrix
stages of aerobic respiration
- glycolysis
- the link reaction
- the krebs cycle
- oxidative phosphorylation
glycolysis makes…
glycolysis makes pyruvate from glucose anaerobically in the cytoplasm
glycolysis- phosphorylation
- glucose is phosphorylated using a phosphate from an ATP molecule
- this creates 1 glucose molecule and 1 ADP molecule
- ATP used to add another phosphate to make hexose bisphosphate
- hexose bisphosphate is split into 2 molecules of triose phosphate
glycolysis- oxidation
- triose phosphate is oxidised forming 2 molecules of pyruvate
- NAD collects H+ ions forming 2 NADH
- 4ATP are produced but net gain is 2ATP
products of glycolysis - aerobic respiration
2NADH-> to oxidative phosphorylation
2 pyruvate-> actively transported into the matrix for use in the link reaction
2ATP-> used for energy
anaerobic respiration- alcoholic fermentation
- occurs in yeast and plants
- pyruvate turns to ethanal producing CO2
- ethanal to ethanol producing NAD from NADH
anaerobic respiration- lactate fermentation
- animal cells and some bacteria
- pyruvate turns to lactate producing NAD from NADH
- regeneration of NAD means that glycolysis can continue even when there isn’t much oxygen
the link reaction
- converts pyruvate produced in glycolysis to acetyl coenzyme A
- pyruvate is decarboxylated, 1C removed as CO2
- at the same time, pyruvate is oxidised to form acetate and NAD is reduced to form NADH
- acetate is combined with coenzyme A to form acetyl coenzyme A
- no ATP produced
- occurs in mitochondria
how many times does the link reaction occur per glucose?
2 pyruvate made per glucose
products of the link reaction
2 acetyl coenzyme A-> to the krebs cycle
2 CO2 -> released as a waste product
2 NADH -> to oxidative phosphorylation
the krebs cycle
- produces coenzymes and ATP
- occurs in matrix
- cycle happens once for every pyruvate molecule
krebs cycle step 1
- formation of citrate
- acetyl coenzyme A from link combined with oxaloacetate to form citrate
- coenzyme A goes back to the link to be used again
krebs cycle step 2
- formation of 5C compound
- citrate is converted to a 5C compound
- decarboxylation occurs and CO2 is removed
- dehydrogenation occurs, the hydrogen is used to produce NADH from NAD
krebs cycle step 3
- regeneration of oxaloacetate
- 5 carbon molecule is converted to oxaloacetate
- 1 molecule of FADH produced
- 2 molecules of NADH produced
- ATP is produced by direct transfer of a Pi from an intermediate to ADP
- when a phosphate group is directly transferred from one molecule to another, it’s called substrate level phosphorylation
- citrate is now oxaloacetate