Respiration Flashcards

1
Q

Where does the LR + krebs cycle take place

A

Mitochondrial matrix

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2
Q

Which processes produce ATP in respiration

A

Glycolysis
Krebs cycle

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3
Q

Each LR yields

A

1X acetylcoA
1X CO2 (decarboxylation- decarboxylase)
1X reduced NAD
since glucose produces 2X pyruvate (3C) all of this will be produced twice

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4
Q

What does coenzyme do

A

Helps bind acetate (2C) to the 4C oxaloacetate in krebs cycle

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5
Q

What does each krebs cycle yield

A

2 X CO2
1 X ATP
3 X reduced NAD+1FAD
* 1 molecule of glucose produces 2 X acetylcoA so KREBS CYCLE HAPPENS TWICE

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6
Q

alternative respiratory substrates

A

Lipids hydrolysed into fatty acids and glycerol
Glycerol phosphorylated and converted into TP - which enter glycolysis + krebs cycle
Fatty acids broken down into 2C fragments and converted into Acetylco A
Oxidation of fatty acids yields many hydrogen atoms to reduce NAD/FAD for oxidative phosphorylation of ATP

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7
Q

Explain how the amount of ATP increased by reactions occurring inside a mitochondrion

A

oxidation of pyruvate
Substrate level production of ATP
Production of reduced NAD/FAD
In matrix of mitochondria
Electrons fed into ETC
Through a series of redox reactions releasing energy
Protons pumped into inter membrane space
Protons flow back through ATP synthase
Synthesising ATP
From ADP + Pi

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8
Q

Describe the roles of coenzymes and carrier proteins in the synthesis of ATP

A

REDUCED NAD/FAD
electrons transferred from coenzyme to coenzyme
Energy released as electrons travel along the ETC thru redox reactions
Energy used to synthesise ATP from ADP + Pi
Using ATP synthase
Protons pumped into inter membrane space
Protons diffuse back through ATP synthase

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9
Q

Describe the events of oxidative phosphorylation

A

NAD/FAD reduced
ETC on cristae
Electrons transferred from coenzyme to coenzyme thru series of redox reactions
Energy released as electrons move along
Protons pumped into intermembrane space
Protons flow back through ATP SYNTHASE
Energy used to synthesise ATP from ADP +Pi

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10
Q

How much ATP does anaerobic respiration produce

A

2 X ATP
Per glucose

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11
Q

How much Atp does aerobic respiration produce per glucose

A

38

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12
Q

Glycolysis

A

Glucose phosphorylated
This required hydrolysis of 2 ATP TO ADP +Pi to provide phosphates
Glucose phosphate splits into 2 X TP
TP oxidised into pyruvate
Loss of hydrogen (via dehydrogenase)
Reduces NAD
Net gain of 2 ATP produced via SLP

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13
Q

Water is a waste product of aerobic respiration. Describe how water formed

A

Oxygen final electron acceptor
Combines with electrons and protons to form water

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14
Q

Explain why its important for plants to produce ATP during respiration in addition to photosynthesis

A

Plants cannot photosynthesise in the dark
Some tissues unable to photosynthesise (roots)
ATP cannot be stored
Plants use more ATP than produces in photosynthesis
ATP used for processes such as AT

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15
Q

Some lactate converted back into pyruvate. Suggest advantage

A

Pyruvate used in aerobic respiration
Less lactic acid build up so less fatigue/cramps

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16
Q

Describe how acetylcoenzyme A is formed in the LR

A

Oxidation of pyruvate
Addition of coenzyme A

17
Q

Explain why converting pyruvate to lactate allowed continued production of ATP during anaerobic respiration

A

REDUCED NAD oxidised
Pyruvate takes the hydrogen
Regenerates NAD
Allows glycolysis to continue

18
Q

Describe how NAD regenerated in anaerobic respiration in yeast cells

A

Formed when REDUCED NAD is used to reduce pyruvate to ethanol + CO2

19
Q

Give 2 reasons why respirometer left for 10mins in water bath

A

Equilibrium reached
Allow for pressure change in apparatus
Allow respiration rate in seeds to stabilise

20
Q

Malonate inhibits a reaction in krebs cycle
Explain why malonate would decrease uptake of oxygen in respiring cell

A

Less reduced NAD
Oxygen final electron acceptor

21
Q

Is glycolysis aerobic or anerobic

A

Anaerobic

22
Q

Mitochondria in muscles have many cristae
Explain advantage

A

Larger SA for ETC
Provides more ATP for muscle contraction

23
Q

Oxidative phosphorylation

A

Atp generated directly through the chemical energy released when a reduced hydrogen carrier or co enzyme (NADH2 / FADH2) oxidised at ETC

24
Q

What does glycolysis yield

A

2 NET Atp

2 Reduced NAD

2 PYRUVATE

25
Q

Deacribe how oxidation takes place in glycolysis + krebs cycle

A

Removal of hydrogen
By dehydrogenase
Reduced NAD FORMED
in krebbs cycle
REDUCED FAD FORMED TO

26
Q

Aerobic respiration produces more ATP PER MOLECULE of glucose than anaerobic respiration
Why

A

Oxygen final electron acceptor
Oxidative phosphorylation occurs in aerobic respiration
Anaerobic respiration only glycolysis
No LR