Respiration

Question 1

what is the site of glycolysis

Answer 1

cytoplasm of cells

Question 2

State the different stages of glycolysis

Answer 2

1. phosphorylation
2. lysis
3. phosphorylation
4. dehydrogenation & formation

Question 3

Explain what happens in phosphorylation and lysis

Answer 3

1. hexose bisphosphate(6C) is produced when 2 Phosphate groups are attached to hexose.2x Pi from 2 units of ATP
2. lysis- hexose bisphosphate is unstable so breaks down into 2x triose phosphate (3C)

Question 4

Explain what happens in phosphorylation and dehydrogenation ATP

Answer 4

1. Pi group attache to the 2xtriose phosphate forming triose bisphosphate.
2. 2x triose bisphosphate oxidised by the removal hydrogen. Forming pyruvate.

Question 5

What is the role of NAD in glycolysis

Answer 5

In dehydrogenation of ATP, NAD accepts th H removed from triose bisphosphate. So NAD becomes reduced.

Question 6

Explain how substrate level phosphorylation plays occurs in glycolysis

Answer 6

ATP formed by transfer of phosphate group from a phosphorylated intermediate (triose bisphosphate)

Question 7

Explain how substrate level phosphorylation is demonstrated in glycolysis

Answer 7

ATP formed by transfer of phosphate group from a phosphorylated intermediate (triose bisphosphate)

Question 8

Define substrate level phosphorylation

Answer 8

Formation of ATP w/o involvement of electron transport chain

Question 9

What is the net ATP yield and explain

Answer 9

2 molecules of ATP.

4 are produced in the end but 2 are used to prime the process.

Question 10

How many molecules of NAD are produced and what happens to it

Answer 10

• 2 molecules.

- used in oxidative phosphorylation

Question 11

How many molecules of pyruvate are produced and what happens to it

Answer 11

• 2 molecules

- used in the link reaction

Question 12

How many molecules of ATP are produced and what happens to it

Answer 12

• 2 molecules (net)

- Used for energy

Question 13

What is the reaction that links glycolysis and the Krebs cycle called.

How does it link them

Answer 13

the link Reaction

glycolysis occurs in cytoplasm of cell

Question 14

Where does link reaction take place

Answer 14

the mitochondrial matrix

Question 15

where is the ATP produced used in?

Answer 15

1.some ATP used to (actively) transport pyruvate (into the mitochondrion)

2.some ATP used to (actively) transport
H(+) from (reduced) NAD ,
formed in glycolysis / into the mitochondrion