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Resources and Reagents/Acids and Bases/ Quality Management > Resources and Reagents > Flashcards

Resources and Reagents Flashcards

1
Q

Pipette

A

Used for the transfer of a liquid from one container to another
Can be reusable or disposable

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2
Q

Micropipette

A

Used for the measurement of volume in microliters
1-1000 uL

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3
Q

Meniscus

A

The curve at the surface of a liquid in a container

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4
Q

“To Contain” Pipette

A

Calibrated to hold the exact volume of liquid specified

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5
Q

“To Deliver” Pipette

A

Calibrated to deliver the specified volume of liquid when expelled

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6
Q

What are the two types of measuring Pipettes?

A

Serological and Mohr

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7
Q

Serological Pipette

A

Graduated to allow multiple volumes to be delivered
Marks extending to the tip of the pipette

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8
Q

Mohr Pipette

A

Graduating, markings do not extend past the tip
Self draining; controlled delivery

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9
Q

What is a Transfer Pipette?

A

Moving one volume to the next with a higher degree of accuracy

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10
Q

What are the two types of Transfer Pipettes?

A

Volumetric Transfer Pipette and Ostwald-Folin Transfer Pipette

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11
Q

Volumetric Transfer Pipette

A

Indicates one volume

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12
Q

Ostwald-Folin Transfer Pipette

A

Small volumes (blood or serum)

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13
Q

What is the most used pipette in the lab?

A

Automatic Pipette

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14
Q

Why is glass used in the lab?

A

High performance liquid chromatography (HPLC)
Absorbs metal ions

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15
Q

What should be used instead of glassware and why?

A

Plastic
pH’s above 6.0 (alkaline solutions)

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16
Q

What is the most reusable glassware in the lab?

A

Borosilicate glass
Pyrex and Kimax brands

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17
Q

List the containers from most to least accurate

A

Volumetric Flask
Graduated Cylinder
Erlenmeyer Flask
Beaker

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18
Q

What should be done with dirty utensils?

A

Soaked in weak detergent or 10% household bleach

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19
Q

What is centrifugation?

A

Using force to separate the lighter portions of a solution, mixture, or suspension from heavier portions
Measured in revolutions per minute

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20
Q

What are the main types of Centrifuges?

A

Horizontal-head
Angle-head
Ultracentrifuge

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21
Q

Horizontal-Head Centrifuges

A

Swinging bucket type
Serum separator devices

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22
Q

Angle-Head Centrifuge

A

25-52 degree angle

23
Q

Ultracentrifuge

A

Highest speed
Refrigerated

24
Q

When should the Centrifuge be checked and what for?

A

Every 6 months, not differ by more than 5%

25
Q

What are the two kinds of balances?

A

Mechanical (Fulcrum)
Electronic (Electromagnetic force)
Calibrated monthly

26
Q

What is Taring?

A

Obtaining net weight by substracting the container by the total weight of sample and container

27
Q

What is the most commonly used balance in the lab?

A

Single-Pan balance

28
Q

What is the most accurate type of balance?

A

Analytical balance
Accurate to 0.1mg

29
Q

Reagent

A

Compound/mixture used to detect, measure, examine, or produce other substances

30
Q

Reference Material

A

Material with multiple properties to be used for calibration of equipment

31
Q

What is a direct indication of the purity of chemicals/reagents?

A

Quality of the analytical results

32
Q

Which reagents are avaliable?

A

Analytical Reagent (AR)
Ultrapure
Chemically Pure (CP)

33
Q

What needs to be met for a reagent to be described as analytical reagent grade?

A

Meet the specifications of the American Chemical Society

34
Q

Ultrapure Reagent

A

Put through additional purification for specific procedures

35
Q

Chemically Pure Reagent

A

Indicates impurities are not stated and the chemical is not uniform

36
Q

Water Purification

A

Removing contaminants removed from water

37
Q

What is the most frequently used reagent in the lab?

A

Water

38
Q

T/F. Water quality is defined by the purification process used

A

False

39
Q

What processes are used to prepare reagent water?

A

Distillation
Deionization
Reverse Osmosis
Ultrafiltration

40
Q

Distilled Water

A

Purified to remove organic materials
Water is boiled and vaporized

41
Q

Deionization

A

Some or all ions removed
Organic material may be present
Excellent in removing dissolved ionized solids and dissolved gases

42
Q

Reverse Osmosis

A

Water is forced through a semipermeable membrane
Does not remove dissolved gases

43
Q

Ultrafiltration

A

Water passes through a membrane <0.2 mm
Does not effectively remove dissolved ionized solids and gases

44
Q

Ultraviolet Oxidation and Sterilization

A

Oxidation; 185 nm, Sterilization; 254 nm
Sterilization is more commonly used

45
Q

Type III Water

A

Less pure than I and II
Not acceptable for analysis or reagent preparation

46
Q

Type II Water

A

Less pure than I; More pure than III

47
Q

Type I Water

A

Purest water
Requirement for analytical labs
Used for highly sensitive procedures

48
Q

CLRW

A

Used in all quantitative and most qualitative laboratory procedures

49
Q

What must be used in chromosome analyses, HLA testing, and in Vitro fertilization?

A

Special purpose reagent water

50
Q

Calibration

A

Describes the relationship between instrument and concentration
Primary process used to maintain accuracy

51
Q

Calibrator

A

Material used to calibrate an instrument
Used to assess or correct accuracy

52
Q

Control

A

Analyzed for QC purposes
Verify system and accuracy

53
Q

Standards

A

Material with a known concentration to be used in quantitative analysis

Resources and Reagents/Acids and Bases/ Quality Management (3 decks)

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