Resources and Reagents

Question 1

Pipette

Answer 1

Used for the transfer of a liquid from one container to another
Can be reusable or disposable

Question 2

Micropipette

Answer 2

Used for the measurement of volume in microliters
1-1000 uL

Question 3

Meniscus

Answer 3

The curve at the surface of a liquid in a container

Question 4

“To Contain” Pipette

Answer 4

Calibrated to hold the exact volume of liquid specified

Question 5

“To Deliver” Pipette

Answer 5

Calibrated to deliver the specified volume of liquid when expelled

Question 6

What are the two types of measuring Pipettes?

Answer 6

Serological and Mohr

Question 7

Serological Pipette

Answer 7

Graduated to allow multiple volumes to be delivered
Marks extending to the tip of the pipette

Question 8

Mohr Pipette

Answer 8

Graduating, markings do not extend past the tip
Self draining; controlled delivery

Question 9

What is a Transfer Pipette?

Answer 9

Moving one volume to the next with a higher degree of accuracy

Question 10

What are the two types of Transfer Pipettes?

Answer 10

Volumetric Transfer Pipette and Ostwald-Folin Transfer Pipette

Question 11

Volumetric Transfer Pipette

Answer 11

Indicates one volume

Question 12

Ostwald-Folin Transfer Pipette

Answer 12

Small volumes (blood or serum)

Question 13

What is the most used pipette in the lab?

Answer 13

Automatic Pipette

Question 14

Why is glass used in the lab?

Answer 14

High performance liquid chromatography (HPLC)
Absorbs metal ions

Question 15

What should be used instead of glassware and why?

Answer 15

Plastic
pH’s above 6.0 (alkaline solutions)

Question 16

What is the most reusable glassware in the lab?

Answer 16

Borosilicate glass
Pyrex and Kimax brands

Question 17

List the containers from most to least accurate

Answer 17

Volumetric Flask
Graduated Cylinder
Erlenmeyer Flask
Beaker

Question 18

What should be done with dirty utensils?

Answer 18

Soaked in weak detergent or 10% household bleach

Question 19

What is centrifugation?

Answer 19

Using force to separate the lighter portions of a solution, mixture, or suspension from heavier portions
Measured in revolutions per minute

Question 20

What are the main types of Centrifuges?

Answer 20

Horizontal-head
Angle-head
Ultracentrifuge

Question 21

Horizontal-Head Centrifuges

Answer 21

Swinging bucket type
Serum separator devices

Question 22

Angle-Head Centrifuge

Answer 22

25-52 degree angle

Question 23

Ultracentrifuge

Answer 23

Highest speed
Refrigerated

Question 24

When should the Centrifuge be checked and what for?

Answer 24

Every 6 months, not differ by more than 5%

Question 25

What are the two kinds of balances?

Answer 25

Mechanical (Fulcrum) Electronic (Electromagnetic force) Calibrated monthly

Question 26

What is Taring?

Answer 26

Obtaining net weight by substracting the container by the total weight of sample and container

Question 27

What is the most commonly used balance in the lab?

Answer 27

Single-Pan balance

Question 28

What is the most accurate type of balance?

Answer 28

Analytical balance Accurate to 0.1mg

Question 29

Reagent

Answer 29

Compound/mixture used to detect, measure, examine, or produce other substances

Question 30

Reference Material

Answer 30

Material with multiple properties to be used for calibration of equipment

Question 31

What is a direct indication of the purity of chemicals/reagents?

Answer 31

Quality of the analytical results

Question 32

Which reagents are avaliable?

Answer 32

Analytical Reagent (AR) Ultrapure Chemically Pure (CP)

Question 33

What needs to be met for a reagent to be described as analytical reagent grade?

Answer 33

Meet the specifications of the American Chemical Society

Question 34

Ultrapure Reagent

Answer 34

Put through additional purification for specific procedures

Question 35

Chemically Pure Reagent

Answer 35

Indicates impurities are not stated and the chemical is not uniform

Question 36

Water Purification

Answer 36

Removing contaminants removed from water

Question 37

What is the most frequently used reagent in the lab?

Answer 37

Water

Question 38

T/F. Water quality is defined by the purification process used

Answer 38

False

Question 39

What processes are used to prepare reagent water?

Answer 39

Distillation Deionization Reverse Osmosis Ultrafiltration

Question 40

Distilled Water

Answer 40

Purified to remove organic materials Water is boiled and vaporized

Question 41

Deionization

Answer 41

Some or all ions removed Organic material may be present Excellent in removing dissolved ionized solids and dissolved gases

Question 42

Reverse Osmosis

Answer 42

Water is forced through a semipermeable membrane Does not remove dissolved gases

Question 43

Ultrafiltration

Answer 43

Water passes through a membrane <0.2 mm Does not effectively remove dissolved ionized solids and gases

Question 44

Ultraviolet Oxidation and Sterilization

Answer 44

Oxidation; 185 nm, Sterilization; 254 nm Sterilization is more commonly used

Question 45

Type III Water

Answer 45

Less pure than I and II Not acceptable for analysis or reagent preparation

Question 46

Type II Water

Answer 46

Less pure than I; More pure than III

Question 47

Type I Water

Answer 47

Purest water Requirement for analytical labs Used for highly sensitive procedures

Question 48

CLRW

Answer 48

Used in all quantitative and most qualitative laboratory procedures

Question 49

What must be used in chromosome analyses, HLA testing, and in Vitro fertilization?

Answer 49

Special purpose reagent water

Question 50

Calibration

Answer 50

Describes the relationship between instrument and concentration Primary process used to maintain accuracy

Question 51

Calibrator

Answer 51

Material used to calibrate an instrument Used to assess or correct accuracy

Question 52

Control

Answer 52

Analyzed for QC purposes Verify system and accuracy

Question 53

Standards

Answer 53

Material with a known concentration to be used in quantitative analysis