Research Paper on human cytomegalovirus

Question 1

What does UL32 gene encode?

Answer 1

pp150: late protein involved in late stages of infectious cycle; implicated in interaction with capsids

Question 2

What are the two debated methods of CMV egress?

Answer 2

1. CMV acquires an envelope from outer nuclear membrane, followed by vesicular transport to secretory pathway. 2. CMV acquires envelope from inner nuclear membrane, fuses and release from outer nuclear membrane releasing NC into cytoplasm where egress continues

Question 3

Accumulation of dense bodies of tegument protein occurs next to the nucleus in a

Answer 3

cytoplasmic inclusion

Question 4

pp150 is a major component of____ and ____ but not of ____

Answer 4

mature virions and noninfectious enveloped particles; dense bodies

Question 5

Cell to cell transfer of _____ is independent of pp150, showing that release of ____ is distinct from that of virions

Answer 5

pp65; dense bodies

Question 6

What was a MAb 36-4monoclonal antibody used for?

Answer 6

detecting pp150

Question 7

what time of cells were used?

Answer 7

human foreskin fibroblasts

Question 8

What enzyme was used to digest DNA and confirm that the UL32 was missing?

Answer 8

HindIII restriction endonuclease

Question 9

MCP and gB accumulated more intensely in which cells?

Answer 9

Delt UL32-BAC, and then UL32-HFF cells due to low protein expression