required practical 6:aseptic techniques

Question 1

what aseptic techniques kill microbes?

Answer 1

1. washing hands
2. disinfect bench/cloth
3. flame instrument/equipment

Question 2

what aseptic techniques prevent contamination/entry of microbes?

Answer 2

1. wearing gloves and/or an apron
2. disinfect bench/cloth
3. flame any instruments/equipment
4. lift the lid slightly when transferring

Question 3

what is the purpose of having a burning bunsen close by?

Answer 3

to create an upward current of air

Question 4

why would you produce a lawn plate/key step in producing one?

Answer 4

to evenly spread the microbes

Question 5

what is the purpose of flaming the neck of the bottle and replacing the lid?

Answer 5

to kill the microbes that can harm you+culture

Question 6

“1.hold the lid of the plate in place with 4 pieces of tape. place your plate upside down ready to incubate 2.at 20 degrees for 24 hours” what is the purpose of this?

Answer 6

1. to create anaerobic conditions for pathogenic growth

2. encourages pathogenic growth

Question 7

why is agar gel boiled prior to pouring plate?

Answer 7

to remove all the microorganisms present

Question 8

what is the purpose of transferring the same volume of liquid culture onto each agar plate?

Answer 8

so the same number of bacteria is transferred to allow for comparison