Required Practical 2

Question 1

Describe how temporary mounts are made

Answer 1

Put 5a of onion root onto slide and cover with toluideine blue stain and lower cover slip

Question 2

Describe how scientists could have used the temporary mounts of leaves to determine the mean number of cholorplasts in mesophyll cells of a leaf

Answer 2

Count all the cells in the field of view.
Count the number of chloroplasts.
Divide the number of chloroplast by number of cells

Question 3

The student cut thin sections of tissue to view with optical microscope. Explain why it was important that the sections were thin.

Answer 3

To allow light to pass through
Single few layer of cells to be viewed

Question 4

When the student was making their microscope slides, they were told to ensure they did not move the over slip sideways. Explain why

Answer 4

To prevent chromosomes from breaking and cells rolling together

Question 5

Describe how temporary mounts are made

Answer 5

Create a thin layer
State the specimen
Push down on or blot t using the cover slip

Question 6

Describe and explain what the student should have done when counting cells to make sure that the kitotic index he obtained for this root tip was accurate

Answer 6

Large field of view
Representative sample
Repeat the count