require practical 6: aseptic techniques

Question 1

examples of aseptic techniques used

Answer 1

wash hands with soap, disinfect surfaces to kill microbes and prevent contamination.
sterilise pipette, spreader and agar plate to kill microbes.
flame neck of the bottle of bacteria to kill microbes.
have a bunsen burner close to keep air-borne bacteria away.
minimise opening lid of agar plate to prevent entry of microbes.

Question 2

describe method to investigate effect of antimicrobial substances

Answer 2

prepare surface using aseptic techniques.
use a sterile pipette to transfer bacteria from broth to agar plate
use sterile spreader to evenly spread bacteria
place discs on agar soaked in different types of antimicrobials
tape lid and incubate
measure diameter of inhibition zone