Reproduction, hormones and plant hormones Flashcards
(47 cards)
Asexual reproduction
one parent
No meiosis- only mitosis in an asexual life cycle
Offspring are genetically identical to each other and to the parent
No genetic variation is generated as existing gene combinations pass unchanged from generation to generation
Advantage: in an unchanging environment, well adapted parents produce well adapted offspring
Sexual reproduction
two parents (male and female)
Meiosis is part of every sexual life cycle
Offspring are genetically different from each other and from their parents
Genetic variation is generated as new gene combinations are produced in every generation
Advantage: species can evolve in a changing environment, as offspring can be better adapted than their parents
The role of meiosis in sexual reproduction
Meiosis and fusion of gametes have different roles
Fusion of gametes: a male and a female gamete join together to form a new individual. This brings alleles together in new combinations. Fusion of gametes is also referred to as fertilisation. It doubles the number of chromosomes each time it occurs.
Meiosis: one diploid nucleus divides to form four haploid nuclei. This breaks up parental combinations of genes, allowing new combinations to form when gametes fuse. By halving the number of chromosomes, meiosis reverses the doubling that is caused by the fusion of gametes, so there is no overall change in the number of chromosomes over a sexual life cycle
Male gametes
Motility of gamete: male gametes travel to the female gamete
Size of gamete: smaller, allowing faster movement
food reserves: less- only enough for the gamete
numbers produced: more- often very large numbers
Female gametes
Motility of gamete: female gametes are non-motile
Size of gamete: larger due to stores of food reserves
Food reserves: more- for embryo development
Numbers produced: few- sometimes only one
The male reproductive system
- erectile tissue- fills with blood to enlarge and harden the penis
- penis- penetrates the vagina so semen can be ejaculated near the cervix
- urethra- transfers semen during ejaculation and urine during urination
- testis- produces sperm and testosterone
- sperm duct- transfers sperm during ejaculation
- seminal vesicle/prostate gland- secrete alkaline fluid, proteins and fructose which is added to sperm to make semen
- epididymis- stores sperm until ejaculation
- scrotum- holds testes at lower than core body temperature to promote sperm development
The female reproductive system
- ovary- produces eggs, oestradiol and progesterone
- uterus- provides for the needs of the embryo and then the foetus during pregnancy (protection, supply of food and oxygen, and removal of waste products)
- vulva- protects internal parts of the female reproductive system
- oviduct- collects eggs during ovulation, and provides a site for fertilisation and then moves the embryo to the uterus
- cervix- protects the foetus during pregnancy and then dilates to provide a birth canal
- vagina- stimulates the penis to cause ejaculation and forms the birth canal
The menstrual cycle
The menstrual cycle takes about 28 days and is composed of cycles that happen in the ovaries and uterus.
In the ovarian cycle, follicles start to develop in the ovary, but usually only one completes development and releases its egg on about day 14. After ovulation, the wall of the follicle enlarges and becomes the corpus luteum. If an embryo isn’t present, the corpus luteum breaks down towards the end of the menstrual cycle.
In the uterine cycle, the endometrium becomes thickened, with enhanced blood supply, in preparation for implantation of an embryo. If no embryo is present, the thickening breaks down and passes out of the body. This is menstruation (”period”). As the start of the menstruation is an obvious event, it is used to define day one of the cycle
Four hormones regulate the menstrual cycle by negative feedback and positive feedback. FSH and LH bind to receptors in the membranes of follicle cells. Oestradiol and progesterone affect gene expression and therefore development in the uterus and other parts of the female body.
The role of protein hormones produced by the pituitary gland (menstrual cycle)
- FSH- rises to high levels in the first ten days of the menstrual cycle. During this time it stimulates the development of follicles, each containing an oocyte (egg) and follicular fluid. FSH also stimulates oestradiol secretion by the wall of the developing follicle
- LH rises to a sudden sharp peak on about day 14. It stimulates the maturation of the oocyte and ovulation by bursting of the follicle wall. LH then promotes the development of the corpus luteum, which secretes oestradiol (positive feedback) and progesterone
Role of steroid hormones produced by the follicle wall and corpus luteum in the menstrual cycle
- oestradiol rises to a peak in the second week of the cycle. It stimulates the repair and thickening of the endometrium after menstruation and an increase in FSH receptors to make the follicles more receptive to FSH, which boosts oestradiol production (positive feedback). When it reaches high levels, oestradiol inhibits FSH secretion (negative feedback) and stimulates LH secretion
- Progesterone levels rise following ovulation, reaching a peak and then dropping back to a low level by the end of the menstrual cycle if no embryo is present. Progesterone promotes the thickening and maintenance of the endometrium. It also inhibits FSH and LH secretion by the pituitary gland (negative feedback)
The process of fertilisation (fusion of sperm and egg cell)
Fertilisation is the fusion of a sperm with an egg to form a zygote. Sperm have receptors in their plasma membrane to detect chemicals released by the egg. This enables the sperm to swim towards the egg. Around the egg is a cloud of follicle cells and a layer of glycoproteins (zona pellucida)
The sperm push between the cells and digest a route through the glycoproteins to reach the plasma membrane of the egg cell. The sperms plasma membrane has proteins that bind to the egg cell’s plasma membrane. The first sperm that manages to reach the egg binds to it and the membranes of the sperm and egg fuse together. The sperm nucleus then enters the egg cell. This is the moment of fertilisation. Immediately afterwards the layer of glycoprotein hardens to prevent further sperm entry
The sperm tail does not penetrate the egg during fertilisation or is broken down inside the zygote. Sperm mitochondria may also penetrate, but they are destroyed by the egg cell.
The nuclei from the sperm and egg remain separate until the zygotes first mitosis. Then both nuclear membranes break down, releasing 23 chromosomes from each nucleus. These chromosomes, half from the mother and half from the father, participate jointly in mitosis, using the same spindle of microtubules. Two genetically identical nuclei are produced, each with 46 chromosomes.
Why do male and female gametes need to be differently adapted
Male gametes travel to female gametes and because of this they have very different adaptations. Male gametes in all animals and some plants are motile- they can swim. The faster they swim, the more chance of reaching the egg and fertilising it, so small size and an efficient propulsion system are needed. Male gametes are produced in larger numbers than female, to increase the chance of one of them fertilising an egg.
In humans (and other species) female gametes are much larger as they contain nearly all the food reserves for the early development of the embryo. Because of the large investment of resources, smaller numbers of female than male gametes are produced. Female gametes have a mechanism for allowing one sperm to penetrate, but not more.
Ovum adaptations
- haploid nucleus- contains the 23 chromosomes that are passed from mother to offspring
- two centrioles
- first polar body- not needed so breaks down
- plasma membrane
- layer of follicle cells (corona radiata)
- zona pellucida- protects the egg cell and restricts the entry of sperm
- cortical granules- harden the zona pellucida to prevent multiple fertilisation (polyspermy)
- cytoplasm/ yolk- contains droplets of fat and other nutrients needed during early stages of embryonic development
Sperm (spermatozoa) adaptations
- acrosome- contains enzymes which digest the zona pellucida around the egg
- plasma membrane
- centriole
- mid-peice with helical mitochondria- produce ATP by aerobic respiration to supply energy for swimming and other processes in the sperm
- haploid nucleus- contains the 23 chromosomes that are passed from father to offspring
- tail/flagella- provides the propulsion that allows the sperm to swim up the vagina, uterus and oviduct until it reaches the egg
- protein fibres to strengthen the tail
- microtubules in a 9+2 array- make sperm tail beat from side to side and generate the forces which propel the sperm
Barriers to prevent polyspermy (Multiple sperm fusing with the egg)
Fusion of more than one sperm with an egg is called polyspermy. Structure in the sperm and the egg make polyspermy very infrequent. A coat of glycoproteins (zona pellucida) surrounds and protects the egg. When sperm make contact with the zona pellucida, they release the contents of their acrosome. This is the acrosome reaction. Enzymes from the acrosome digest a small region of the zona pellucida, allowing the sperm to penetrate and reach the egg.
When the first sperm has fused with the egg, the many cortical granules (vesicles) near the eggs plasma membrane release their contents by exocytosis. This is the cortical reaction. The enzymes that are released toughen the zona pellucida making it very difficult for any more sperm to penetrate it. The enzymes also change specific glycoproteins in the zona pellucida to which sperm bind, so that this can no longer happen.
what is IVF?
Natural fertilisation is in vivo, as it occurs inside the body. Fertilisation can also happen outside the body in dishes in the laboratory. This is in vitro fertilisation, usually abbreviated to IVF. The procedure has been used extensively to overcome male/ female fertility problems. There are various protocols for IVF.
What are the stages of IVF
- down-regulation: every day for two weeks, the woman has an injection or nasal spray containing a drug to stop the pituitary gland from secreting LH or FSH. Secretion of oestradiol and progesterone therefore also stops, pausing the womans normal menstrual cycle, allowing doctors to control the timing and amount of egg production
- FSH injections- intramuscular injections of FSH are given daily for 7-12 days, to stimulate follicles to develop. The aim is to generate very high FSH concentration, resulting in far more follicles than normal (superovulation). The aim is 8-15 follicles, each containing an egg.
- hCG injection- when the follicles are 18mm in diameter, they are stimulated to mature by an injection of human chorionic gonadotropin (hCG). This is the hormone embryos secrete to signal to their mother that they are present
- Egg collection- this is a minor surgical procedure that takes around 20 minutes. A micropipette mounted on an ultrasound scanner is used to draw the eggs out of the follicles. Egg collection is done about a day and a half after hCG injection.
- Fertilisation- each egg is mixed with 50,000-100,000 sperm cells in sterile conditions in a shallow dish, which is incubated at 37 degrees celsius until the next day
- Embryo transfer- one or more embryos are placed in the uterus when they are about 48 hours old. A progesterone tablet is placed in the vagina, to ensure that the uterus lining is maintained. If the embryos implant and continue to grow, the pregnancy that follows is no different to a normal pregnancy that began by natural conception.
What is hCG (human chorionic gonadotropin)?
The hormone human chorionic gonadotropin (hCG) is a medium- sized protein. hCG is produced by the trophoblast cells in the blastocyst and subsequent embryonic stages, so it is an early indicator of the presence of an embryo. Later in pregnancy, cells in the planceta continue the secretion of hCG.
How do pregnancy tests work?
Tests for pregnancy are based on the detection of hCG in a womans urine. A test stick has an absorbent strip inside a plastic casing. There are three types of monoclonal antibody in the strip.
A urine sample is placed on the strip where it sticks out at one end. The urine is drawn through the strip by capillary action. it first meets antibody A and has a molecule of blue dye detached. Any hCG molecules in the urine bind to this antibody. Antibody A with or without bound hCG, moves on with the urine along the strip. Next is a band of immobilised antibody B which binds to antibody A which have hCG bound, creating a blue band which indicates pregnancy. Molecules of antibody A without hCG move along the strip and reach a band of IgG antibody, to which they bind, creating a blue band which indicates the test has worked.
What is gametogenesis?
Gameteogenesis is the production of gametes. Male gametes are sperm (spermatozoa) and female gametes are eggs (oocytes). Spermatogenesis happens in the testes and oogenesis in the ovaries. The four stages in both types of gametogenesis are mitosis, cell growth, two divisions of meiosis and then differentiation.
What is spermatogenesis?
Spermatogenesis occurs in the seminiferous tubules of the testes, which are lined with a germinal epithelium.
Spermatogonia (diploid germ cells) divide by mitosis to produce more spermatogonia.
Some spermatogonia grow into primary spermatocytes, which undergo meiosis I to form two secondary spermatocytes.
Secondary spermatocytes undergo meiosis II to form four haploid spermatids.
Sertoli cells provide nourishment and support during development.
Spermatids undergo differentiation (spermiogenesis) to become mature spermatozoa.
The developing sperm cells are gradually pushed toward the lumen of the seminiferous tubule.
Leydig (interstitial) cells, located between tubules, produce testosterone, which stimulates spermatogenesis.
What is oogenesis?
Oogenesis occurs in the ovaries, starting in the germinal epithelium. During foetal development, these cells divide by mitosis and migrate into the cortex. By 4–5 months, they grow and start meiosis, but pause in prophase I. At birth, a female has primary follicles, each containing a cell arrested in meiosis I.
At puberty, during each menstrual cycle, some primary follicles begin development, but usually only one matures and ovulates. Meiosis I completes, producing a large secondary oocyte and a small first polar body (which degenerates). The secondary oocyte begins meiosis II but pauses in metaphase II. Meiosis II only completes if fertilization occurs, forming an ovum and a second polar body (which also degenerates).
Development of a blastocyst
The zygote produced by fertilisation in the oviduct divides by mitosis to form a 2-cell embryo. Further rounds of the cell cycle double the number of cells about every 18 hours. Because the egg has a large amount of cytoplasm, these early rounds of division can happen without any cell growth, so cell size decreases. Initially the embryo is a solid ball of cells. When it is 6 or 7 days old, it has changed into a hollow ball, due to unequal divisions and cell migration. This embryonic stage is the blastocyst. It has about 250 cells and is 200 micrometres in diameter.
While the embryo is developing it is wafted down to the oviduct by cilia in the oviduct wall. The embryo arrives in the uterus when it is about 7 days old and has become a blastocyst. The embryo has used up the food reserves of the egg by this stage, so it must obtain supplies from its mother. Cells in the outer layer (trophoblast) of the blastocyst secrete enzymes that loosen the connections between cells in the lining of the uterus wall (endometrium). This allows the blastocyst to penetrate the uterus wall and become implanted. If implantation does not occur, the embryo is not supplied with enough food and the pregnancy fails.
What is the placenta?
The placenta is a disc-shaped structure, embedded in the uterus wall. It’s development begins about four weeks after conception in humans. The basic functional unit is a finger-like piece of foetal tissue called a placental villus. The size of the placenta and the number of villi grow larger during the pregnancy, so that the surface area matches the increasing demands of the foetus for exchange of materials with the mother. Maternal blood flows in spaces around the villi (intervillous spaces). This is a very unusual type of circulation as elsewhere blood is almost always retained in blood vessels. Foetal blood is pumped to the placenta through arteries in the umbilical corod by the foetal heart and then passes through a dense network of blood capillaries close to the surface of each villus.