replicative cycle of variant phage e.g. T4

Question 1

variant phage only has a

Answer 1

lytic cycle

Question 2

attachment

Answer 2

t4 tail fibres bind to LPS and ompC on surface of E. coli. base plate comes in contact w/cell surface and sheath becomes shorter pushing the central tube to cell wall. base plate has T4 lysozme which helps the central tube to get through the peptidoglycan. DNA passes into cytoplasm. rest of phage remains outside

Question 3

attacmhent contd

Answer 3

components are replicated they self assemble. late in infection a T4 enzyme called hopi forms holes in the PM. lysozyme passes through the holes and opens up to peptidoglycan. cell lyses releases the phage