B HIV1 structure

Question 1

genome

Answer 1

it is diploid. 2 copies of +ive strand RNA are cap and polyadenylated. each copy includes all genes required for replication. sequence of gene may not be identical

Question 2

how are the 2 copies of +ive RNA strand held together

Answer 2

regions where they are held together have H bondings

Question 3

each +ive strand RNA is coated w/

Answer 3

a nucleocapsid protein called NC. NC binds specifically near the 5’ end of genome and non specifically to the rest

Question 4

how does NC ensure retroviral not cellular mRNA will be packaged in the virion

Answer 4

NC binds to specific packaging sequence on HIV +strand RNA. HIV recognizes NC coated RNA and packages that

Question 5

tRNA

Answer 5

non random partially unwound tRNA is h-bonded to each copy of genome near the 5’ end. binds to primer binding site

Question 6

capsid

Answer 6

composed w/multiple subunits of capsid protein called CA. in HIV capsize is bell shaped, other retroviruses it is icosahedral. inside is genome coated w/NC and bound to tRNA. also multiply copies of reverse transcriptase integrase and a protease

Question 7

envelope

Answer 7

one spike protein. they are trimers each monomer is composed of 2 subunits. SU stands for surface protein, contains site that binds to receptor and corrector. TM is transmembrane protein, has a fusion peptide at its N terminus

Question 8

to which part it the inner domain of TM linked to

Answer 8

matrix protein which is MA