Replication Flashcards
1
Q
What does dnaA do
A
Tag origin & initiate replication
2
Q
Why dna synth 5-3
A
Dna pol only add at 3 end
3
Q
Dna a helicase
A
Unwind
4
Q
Topoisonerase (gyrase)
A
Relive supercoiling
5
Q
SSB pro
A
Bind to exposed bases
6
Q
Primase
A
Synth 10nt rna primer
7
Q
Necessities for phosphodiester bond
A
Trinucleotides
OH on 3’C
8
Q
Ligase need
A
Atp
9
Q
Pol3
A
Synth
10
Q
Pol1
A
(Repair) remove primer & replace with dna
11
Q
Exonuclease
A
Lag proofread
Lead remove primer
12
Q
Ligase
A
Seal nicks
13
Q
Packing stages
A
Nucleosomes
30nm fibre
Scaffold assoc
14
Q
What is origin of replication
A
Point / particular base seq