Replication Flashcards

1
Q

What does dnaA do

A

Tag origin & initiate replication

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2
Q

Why dna synth 5-3

A

Dna pol only add at 3 end

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3
Q

Dna a helicase

A

Unwind

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4
Q

Topoisonerase (gyrase)

A

Relive supercoiling

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5
Q

SSB pro

A

Bind to exposed bases

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6
Q

Primase

A

Synth 10nt rna primer

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7
Q

Necessities for phosphodiester bond

A

Trinucleotides

OH on 3’C

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8
Q

Ligase need

A

Atp

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9
Q

Pol3

A

Synth

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10
Q

Pol1

A

(Repair) remove primer & replace with dna

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11
Q

Exonuclease

A

Lag proofread

Lead remove primer

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12
Q

Ligase

A

Seal nicks

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13
Q

Packing stages

A

Nucleosomes
30nm fibre
Scaffold assoc

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14
Q

What is origin of replication

A

Point / particular base seq

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