Enzymes

Question 1

On graph what is x int and y int

Answer 1

X - -1/km

Y- 1/vmax

Question 2

Issues with graph

Answer 2

Error prone due to clustering of data points at higher s

Question 3

What is km

Answer 3

Measure of s conc for half v max/ effective catalysis

Question 4

3 catalyst

Answer 4

Orientation
Covalent
Acid/base

Question 5

Kinase

Answer 5

Transfer phosphate group

Question 6

Competitive rev eg

Answer 6

Succinate dehydrogenase (malonate v succinate)
Folic acid synth in bacteria

Question 7

Irrev inhib eg

Answer 7

Nerve gas
Pesticide with organophosphate
Arsenic
Snake venom

Question 8

Feedback inhib eg

Answer 8

Bacteria histidine synth, histidine inhib pathway at start

Pyrimidine Nt biosynth

Question 9

Valium v phenobarbital

Answer 9

Valium not deadly in overdose as does not bind to as

Allosteric drug

Question 10

Protein modification modification eg

Answer 10

Kinase action

Question 11

Zymogen

Answer 11

Enzyme precursor/ proenzyme

Question 12

Co enzyme eg

Answer 12

ATP

Modified vitamins

Question 13

Prosthetic group

Answer 13

Tightly bound non pp molecule needed for activity of enz or other pro

Question 14

Metalloenz

Answer 14

Enz need 1+ metal ions for its catalytic fx

Question 15

Mg2+ needed for

Answer 15

Dna pol

Restriction enz

Question 16

Isoenz

Answer 16

Isomers of enz different aa seq but same fx

Allow fine tune for met cond/ dev stage

Question 17

Alloenz

Answer 17

Product of diff alleles of same gene

Question 18

Hecokinase 1 v VI

Answer 18

1 low km inhib by glucose (supplies E at low glucose conc (found everywhere)
4 (aka glucokinase) high km not inhib by glucose (liver pancreas brain)
4 Make glycogen in liver at high conc

Question 19

What graph for km and vmax

Answer 19

1/v against 1/s conc