Replication Flashcards
What is the purpose of min proteins in replication?
they are negative regulators, therefore they inhibit the process of the FitZ cleaving the two daughter cells apart in cellular division. They are one of two factors in cytokinesis in bacteria.
Other than min proteins, what other factor contributes to inhibiting the cells from invaginating?
the nucleoid being in the center (nucleoid occlusion proteins)
What is the name of the proteins in the nucleoid that stops FitZ from forming in the center?
SimA
Describe the Helmstetter and Cooper Experiment of 1968
Find DNA content at cells at different stages of the cell cycle. They labeled DNA with radioactive nucleosides to get a population that are all the same age in the same replication state.
What does “I” mean in regards to replication?
Time from last round of replication until a new round begins
What does “C” mean regarding replication?
time it takes to replicate the CHROMOSOME
What does “D” mean regarding replication?
time between replication termination and cell DIVISION
In conclusion of the experiment, what factors are constant?
C and D. I is the only one that varies.
Initiation is also regulated by (think of what the black circles stood for)
methylation
What is hemimthylated DNA?
when only one of the complementary strands is methylated
What regions of DNA are involved in initiation of repliation?
helicase, primase, ssb, dna polymerase
Describe termination
ter regions act as gates to trap and gatekeep the replication forks until the tus proteins get there to knock off the replisome
can recombination occur in the termination region?
ye
what is a chromosomal dimer
2 circular daughter dna are still joined together
how are chromosomal dimers resolved?
xer recombination systems and the dif site located in the ter region. Dependent on the formation of division seotum via fitsk interaction
