Replication Flashcards
What direction is DNA synthesized?
5-3 prime direction
What kind of process is replication?
Semi-conservative
What enzyme relaxes the supercoiling in front of the replication fork?
Topoisomerase
What enzyme unzips the DNA strand?
Helicase
What is the place where DNA opens up called?
Replication fork
What things stabilize the parental strand?
Single-stranded binding proteins (SSB Proteins)
What is a type of RNA polymerase that initiates DNA replication by synthesizing a short RNA primer?
Primase
What is a short stretch of D/RNA that nucleotides bonded to the complimentary strand?
Primer
What can DNA NOT begin without?
Primer
What does DNA polymerase do?
Synthesizes new DNA strands continuously on leading strand and discontinuously on lagging strand
Which DNA polymerase only reads from 3-5 direction?
DNA polymerase III
Which DNA polymerase removes the RNA primer?
DNA Polymerase I
Which DNA polymerase is mainly used for DNA repair?
DNA Polymerase II
What does the Ligase do?
It binds Okazaki fragments to lagging strand
What are the 3 stages or replication?
Initiation
Elongation
Termination