remedial monday Flashcards
this type of culture medium contains inhibitory agents for isolation of specific microorganism groups
SELECTIVE MEDIUM
what is the optimal cultivation time for growing microbes
18-24 hours
optimal temperature for growing bacteria
35-37 C
this is the cause of the acid fastness of acid fast bacteria
mycolic acid
this microscope is used to view ultra structures of a specimen
TRANSMISSION ELECTRON MICROSCOPE
this microscope is used to observe the detailed surface structures of microorganisms
scanning electron microscope
this technique in AST uses an antibiotic disk on MHA
kirby-bauer technique
this is a national resource for molecular biology information
NCBI
this virulence factor of s.aureus activates complement, IL-1, and is chemotactic to PMNs
Peptidoglycan
this virulence factor of s.aureus has affinity to the Fc receptor of IgG and complement
Protein A
this cytotoxin in s.aureus is associated with panton valentin leukocidin
cytotoxin gamma
this toxin is responsible for toxic shock syndrome in s.aureus infection
TSST-1 / ENTEROTOXIN F
this virulence factor is also known as spreading factor in s.aureus
hyaluronidase
this virulence factor in s. aureus is lethal to PMNs and responsible for severe cutaneous infections and necrotizing pneumonia
Panton Valentin Leukocidin
bullous exfoliative dermatitis that can progress to ritter disease
scalded skin syndrome
these s.aureus enterotoxins cause staphylococcal food poisoning
A, D, B
THIS BACTERIA IS A COMMON CAUSE OF HAI UTIs and prosthetic valve endocarditis
s. epidermis
this bacteria is associated with UTIs IN SEXUALLY ACTIVE YOUNG WOMEN
s. saprophyticus
this is the special plating medium for staphylococcal specimens.
5% CNA
this bacteria has colonies that are small tommesium and grayish white that stick to the agar
s. epidermis
this is the selective/inhibitory agent of CNA
naladixic acid
this is the selective agent in Mannitol salt agar
7.5% sodium chloride
mannitol fermenters in MSA produce what color
yellow colonies with yellow halo
positive result of the catalase test
effervescence
you performed an oxidation-fermentation test on a microorganism. The results show a green closed tube but a yellow open tube. What is the organism?
oxidizer
this test yields a blue to purple color after the oxidase enzyme reacts with the oxidase reagent and cytochrome C to create indophenol
Microdase Test
if the DNase test is positive, what result should you expect?
clearing around the growth
what does the tube coagulase test detect?
free coagulase
this staphylococcus species is resistant to novobiocin
s. saphrophyticus
these are the ribosomal RNA sequences for detecting bacteria
16s and 30s
primary mechanism of resistance to beta lactam antibiotics
production of altered PBP
antibiotic used in MRSA screen agar
oxacillin
mechanism in macrolide resistance in bacteria
efflux pump
what does it mean if an organism is capnophilic
requires increased CO2 for growth
this streptococcus is nto considered normal microbiota and can cause rheumatic or scarlet fever
S. Pyogenes / Group A Strep
O2 labile hemolysin responsible for hemolysis under anaerobic conditions
streptolysin O
toxin responsible for non bullous impetigo
epidermolytic toxin
this virulence factor in group A strep causes the molecule to resist phagocytosis and plays a role in adherence to mucosal cells
M Protein
this impetigo does not form a honey coloured crust with no erythema or edema surrounding it
Bullous Impetigo
this illness is characterized by a rash on the face and upper trunk with a strawberry tongue in active infection
scarlet fever
when infected with s.pneumoniae, what is the hallmark color or the sputum
rust colored
this strep group grows in the presence of bile, hydrolyze esculin, grows in 6.5% NaCl and PYR - positive
enterococci
this streptococcal vaccine is recommended for infants, young children, and all adults younger than 65 years
PCV 13
in this disease, the penis has yellowish purulent discharge and dysuria
Gonorrhea
this occurs when meningitis becomes fulminant and spreads rapidly causing disseminated intravascular coagulation, septic shock or haemorrhage in adrenal glands
Waterhouse-Friderichsen Syndrome
common media for initial and general plating of streptococci
5% SBA
this indicates a positive result for s. agalactiae in CAMP test
arrowhead hemolysis
after performing optochin solubility test on streptococci, the result is resistant. What is the speculated microorganism?
Viridians Strep
When a patient undergoes a serologic test for streptococcus infection, what are you searching for?
Anti-Streptolysin O Titer / Streptolysin O
This is the initial plating media for Neisseria species
5% SBA and CAP
This is diagnostic of gonococcal disease when performing direct gram staining of urethral discharge
appearance of gram negative diplococci inside PMNs
this neisseria species ferments glucose and maltose
N. Meningitis
this indicates the presence of cytochrome oxidase activity in microorganism
oxidase test
give the reagent used in the superoxol test.
30% H2O2
default shape of bacteria
cocci
carried by the TOX gene from a beta phage.
Diphtheria Toxin
this is how diphtheria toxin genes are integrated into the chromosome so the bacteria becomes pathogenic
Lysogenic Conversion
this is the effect C. diphtheriae toxin on cells
inhibits protein synthesis
Pharyngitis is often characterized by
exudative pseudomembrane
in tinsdale medium and CTBA, C. diphtheriae produces these characteristics
Brown to Black Colonies with Gray to Brown Halos
this skin test is used to determine the susceptibility of a person to diphtheria toxin
schick test
this virulence factor in listeria enables cell to cell spreading
ACT A
drug of choice for listeria CNS infection
SXT
this technique has samples inoculted in a nutrient broth incubated for several weeks at 4C
Cold enrichment technique
motility of listeria
tumbing end to end motility
listeria growth in SIM
inverted christmas tree/umbrella growth
this serotype causes most foodborne infections
4B
in PCR detection for Listeria, what is targeted by primers
Genes for virulence factors or RNA sub-unit genes
give the clinically significant bacillus species
anthracis, cereus, mycoides, thurginensis
these are painless, small dark areas that appear in the center of the ring with a depressed black necrotic central area
Malignant pustule / black eschar
this is also known as wool sorter’s disease
inhalation anthrax
heat stable proteolysis resistant toxin in B. cereus that causes emetic symptoms
cerulide
toxin that is toxic to insects and nematodes injected into corn
parasporal crystalline inclusions
the size of this gram positive rod is >1 um with no endospore swelling
anthracis
cereus
mycoides
thurginensis
this agar is best and selective for G+ positive bacteria
pea agar
this prevents growth of yeast and filamentous fungi in CNA
amphotericin B
in 5% SBA, small to large colonies with various appearances surrounded with a small zone of beta hemolysis are seen. What is the speculated organism?
Arcanobacterium
this bacteria is often found in clue cells, large squamous epithelia with numerous short rods
G. Vaginalis
what is the hallmark growth of E. rhusiopathae in SIM
Brush growth with blackening in medium
most common drug of choice for G. Vaginalis
Metronidazole
this organism has a greenish metallic sheen in EMB
E. Coli
this microbe is known to be very biochemically inert
shigella
what species are truly pathogenic in family enterobacteriaceae?
salmonella
shigella
yersinia
this is associated with hemorrhagic diarrhea, colitis, and HUS.
E. coli 0157:H7
Case study:
TSIA results were A/A with gas production.
IMV:C results are ++–
What is the bacteria causing symptoms in the patient?
E.coli
