Rehyb HSQ 3K/4K/X

Question 1

How should reagents be stored once a run is stopped?

Answer 1

Wit parafilm at 4deg for up to 48hrs

Question 2

Is HSQ 3k/4k/X rehyb on cBot or onboard?

Answer 2

cBot

Question 3

What step(s) can you rehyb for HSQ 3k/4k/X?

Answer 3

Primer 1 only

Question 4

After stopping a run on HSQ 3k/4k/X, should the HSQ be washed before restarting the rehyb run?

Answer 4

Yes, water wash SBS positions only (40 min)

Question 5

After stopping a run on HSQ 3k/4k/X and rehybing the flowcell, should you “resume run” or start a new run?

Answer 5

You will have to start a new run. There is no resume after a run is stopped. The FC will not be recognized by the HSQ, you wil be allowed to start a new run with the same FC.

Question 6

On HSQ 3k/4k/X, how often to quick format the O:/ and S:/ drives?

Answer 6

Before every run transfer all data to a network location and quickformat the O:/ and S:/ drives

Question 7

What are the (8) steps in a HSQ powercycle?

Answer 7

1) Close HCS
2) Turn off HSQ instrument
3) Turn off computer
4) Wait at least 1 min
5) Turn on computer
6) Turn on HSQ instrument
7) Check for the Donoteject drive
8) Open HCS

Question 8

Are SBS reagents primed using the experimental FC?

Answer 8

No, a used wash FC is to be used for priming reagents.

Question 9

When the lines are separated out, how much volume is expected per line during HSQ 3k/4k/X prime?

Answer 9

1.75 ml

Question 10

List the steps involved in priming before sequencing

Answer 10

Load used FC
Position 2 (PW1) pump
Separate lines
Prime SBS reagents
Load clustered FC
Position 5 (HB2) pump
Sequence