Recombinant DNA technology

Question 1

what makes each organism unique

Answer 1

all the organisms share the same basic structure yet the differentiate due to the nucleotides A,T,G and C sequence

Question 2

recombinant DNA

Answer 2

DNA combined from two different organisms

Question 3

plasmid definition

Answer 3

short circular double stranded DNA found naturally in bacteria and yeast and they are separate from the chromosomal DNA and replicate independently

Question 4

Transformation definition

Answer 4

The ability of the bacteria to take up the DNA from their environment

Question 5

The steps of making a recombinant DNA

Answer 5

1-cutting the gene of interest with a certain restriction enzyme
2- cutting the plasmid with the same restriction enzyme
3- put the plasmid and the gene of interest in a solution with DNA ligase
4- the DNA ligase joins the two together by repairing the sugar-phosphate backbone