electrophorosis Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

Genome

A

Genome is the full set of genetic information that an organism carries

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

the human genome

A

is made up of 3.2 billion base pairs 21 thousand gene and 46 chromosome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

DNA tools

A

tools that cut separate and read DNA sequences which are : restriction enzymes and gel electrophoresis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

restriction enzymes

A

also called endonucleases natural DNA cutting enzymes found in bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

restriction enzymes function

A

the recognize and bind to a specific DNA sequences, thy cut DNA both DNA strands by breaking the sugar phosphate bonds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

blunt end

A

straight-cut DNA that can bind to any DNA with a blunt end

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

sticky end

A

are not cut-straight and can only bind to a complementary sticky ends and are single stranded

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

ecor1

A

a restriction enzyme that produces sticky ends and bint to GAATTC and cut between thwe a and thwe g

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

DNA sign

A

negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

gel electrophoresis

A

a technique that is used to separate DNA fragments based on their size using an electrical current

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

restriction enzyme discovery

A

in the late 1960’s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

the function of restriction enzymes in the bacteria

A

it defends it from viruses by preventing their replication by cutting them into fragments

How well did you know this?
1
Not at all
2
3
4
5
Perfectly