electrophorosis Flashcards
Genome
Genome is the full set of genetic information that an organism carries
the human genome
is made up of 3.2 billion base pairs 21 thousand gene and 46 chromosome
DNA tools
tools that cut separate and read DNA sequences which are : restriction enzymes and gel electrophoresis
restriction enzymes
also called endonucleases natural DNA cutting enzymes found in bacteria
restriction enzymes function
the recognize and bind to a specific DNA sequences, thy cut DNA both DNA strands by breaking the sugar phosphate bonds
blunt end
straight-cut DNA that can bind to any DNA with a blunt end
sticky end
are not cut-straight and can only bind to a complementary sticky ends and are single stranded
ecor1
a restriction enzyme that produces sticky ends and bint to GAATTC and cut between thwe a and thwe g
DNA sign
negative
gel electrophoresis
a technique that is used to separate DNA fragments based on their size using an electrical current
restriction enzyme discovery
in the late 1960’s
the function of restriction enzymes in the bacteria
it defends it from viruses by preventing their replication by cutting them into fragments