Recombinant DNA technology

Question 1

Cloning

Answer 1

making copies of a segment of DNA such as genes

Question 2

Recombinant DNA molecule

Answer 2

splicing a DNA fragment into a cloning vector

Question 3

Vector

Answer 3

DNA molecule capable of replication in a host organism

Question 4

What are the steps of cloning?

Answer 4

1. Isolate DNA
2. Cut DNA with restriction enzymes
3. Insert each piece individually into a vector
4. Introduce recombinant DNA into a host
5. Bacterial cells divide and replicate along with the recombinant DNA

Question 5

What is restriction enzymes function?

Answer 5

Recognize a specific base-pair sequence (restriction site) and cut DNA within that sequence

Question 6

Plasmid

Answer 6

circular extrachromosomal elements that replicate autonomously within cells.

Question 7

Key features of plasmids

Answer 7

1. ori sequence (origin of replication)
2. selectable marker (antibacterial resistance)
3. Unique RE cut sites (polylinker)

Question 8

what is ligase function for RE?

Answer 8

to repair cuts made by restriction enzymes