PCR and molecular markers Flashcards
1
Q
Polymorphism
A
when 2+ variants (alleles) exist for a specific DNA sequence (locus) in the genome
2
Q
Intragenic polymorphism
A
located within the gene
3
Q
intergenic polymorphism
A
located between genes
4
Q
casual polymorphism
A
causes a change in phenotype
5
Q
Molecular marker
A
a polymorphism that can be easily determined via a biotechnology
6
Q
Monomorphic marker
A
both individuals carry the same allele in the same marker (not helpful)
7
Q
Polymorphic marker
A
individuals that carry different alleles at the same marker
8
Q
PCR- Polymerase Chain Reaction
A
method to rapidly produce large amounts of copies of a small sample of DNA
9
Q
Steps of PCR
A
- Melting DNA (denaturation) to single strands
- Primer annealing
- Extension of primers with Taq DNA polymerase
- Repeat
