Recombinant DNA tech

Question 1

Restriction enzymes

Answer 1

-naturally in bacteria
-Active site complementary to range of base sequences
-enzymes cutting at same location leads to blunt end , others cut for staggered ends
-staggered ends palindromic which mean same forward as it is backwards upsidedown

Question 1

reverse transcriptase

Answer 1

-naturally in viruses
-cells that produce protein are selected
-cells need large amounts of mRNA
-reverse transcriptase joins DNA nucleotides with complementary bases
-makes cDNA , single stranded
-To make doubkle stranded need polymerase

positive is its intron free

Question 2

Gene machine

Answer 2

-Examine protein of interest
-check biosafety and ethics
-computer can create oligonucleotides which are overlapping single stranded
-pcr can amplify to double stranded
quick and intron free

Question 3

promoter and terminator region

Answer 3

-promoter added at start which enables transcription to occur , binds RNA polymerase
#-terminator added to end which detatches RNA polymerase stopping transcription