Recombinant DNA

Question 1

Recombinant DNA

Answer 1

DNA composed of genetic material from different sources, often different species

Question 2

Recombinant DNA: Restriction Endonucleases

Answer 2

Enzymes that come from bacteria that cleave interior of DNA at restriction site within target sequence, creating restriction fragments

Question 3

Recombinant DNA: Sequence Specificity

Answer 3

Each type of enzyme always cuts at same spot in same way, producing same restriction fragments

Question 4

Type of Cuts: Staggered Cut

Answer 4

Sticky ends/overhangs: unpaired nucleotides at end of each end –> can only form base pairs with other sticky ends with complementary sequence (specific)

Question 5

Type Cuts: Blunt Ends

Answer 5

No overlap of bases, cut at center of restriction site –> no specificity as any blunt end can combine

Question 6

DNA Ligase

Answer 6

Joins together DNA with matching ends