RECALLS MICRO II Flashcards
CA of Weil’s dse (jaundice)
CM: fletcher’s medium
Leptospira interogans icterohemorrhagica
1️⃣ cause of PID (Pelvic Inflammatory dse)
CA of LGV (Lymphogranuloma inguinale) #1️⃣ cause of NGU (Non-gonococcal Urethritis)
CM: McCoy cells
Chlamydia trachomatis
Can be demonstrated through Giemsa stained blood
Borrelia
CA of WHIPPLE’s dse
Trophyrema whipplei
Causes NGU
Urease +
CM: Sheperd’s mom A7 medium
Ureaplasma urealyticum
Common name: T strain
Vector: TICKS (3)
ERL
Ehlichiosis
RMSF
Lyme’s dse
OX-K +
OX-2 -
OX-19 -
O.tsutsugamushi
scrub typhus
OX-2 : ➕
OX-19:➕
OX-K: ➖
RMSF
Rickettsia rickettsi
Negative for all: OX-2,9,K
Rickettsial pox (Rickettsia akari) Q fever (Coxiella burnetti)
Basic units of fungi
Hyphae
Spores
Has ascospore
Saccharomyces cerevisiae
CA’s or REYE’s syndrome
VZV
INFLUENZA VIRUS
CA of PML (Progressive Multifocal Leukoencephalopathy)
JC VIRUS (polyomaviridae)
CA or SPONGIFORM ENCEPHALOPATHY
PRIONS
Transport of spx for VIRUS:
4’C (ref); if delay of 3days
Transport of virus spx and sputum
4 days delay: -70’C
Used to hold cell culture tube.
Roller drum
Test for the presence of bacteria in milk
Methylene Blue reduction test
When culturing Cryptococcus in SDA, ____ Should NOT be in the medium.
CYCLOHEXIMIDE
Geotrichum candidum colonies appear as:
Rapid, yeastlike growth
Not a routine test in MICRO.
PCR
What does acridine orange stain
NUCLEIC ACIDS OF BACTERIA
Recovery of FUNGI:
Biopsy
CSF
ASPIRATIONS
(Never use swab)
C.diptheriae in Potassium tellurite (color)
Gray black
▪️Common component of the normal floral found on human skin and mucous membrane.
▪️disregarded by physician as a contaminant when found in blood culture.
▪️opp. Pathogen causing human disease: ENDOCARDITIS and SEPSIS
▪️pleomorphic, gram pos rods with single cells, V forms, or Chinese letters
Corynebacterium amycolatum
Preferred concentration technique for parasite ova and cyst
Formalin-ethyl-acetate sedimentation
Staph RESISTANT to 5ug novobiocin
S.saprophyticus
Staph SENSITIVE to 5ug novobiocin
S.epidermidis