RECALLS MICRO II Flashcards
CA of Weil’s dse (jaundice)
CM: fletcher’s medium
Leptospira interogans icterohemorrhagica
1️⃣ cause of PID (Pelvic Inflammatory dse)
CA of LGV (Lymphogranuloma inguinale) #1️⃣ cause of NGU (Non-gonococcal Urethritis)
CM: McCoy cells
Chlamydia trachomatis
Can be demonstrated through Giemsa stained blood
Borrelia
CA of WHIPPLE’s dse
Trophyrema whipplei
Causes NGU
Urease +
CM: Sheperd’s mom A7 medium
Ureaplasma urealyticum
Common name: T strain
Vector: TICKS (3)
ERL
Ehlichiosis
RMSF
Lyme’s dse
OX-K +
OX-2 -
OX-19 -
O.tsutsugamushi
scrub typhus
OX-2 : ➕
OX-19:➕
OX-K: ➖
RMSF
Rickettsia rickettsi
Negative for all: OX-2,9,K
Rickettsial pox (Rickettsia akari) Q fever (Coxiella burnetti)
Basic units of fungi
Hyphae
Spores
Has ascospore
Saccharomyces cerevisiae
CA’s or REYE’s syndrome
VZV
INFLUENZA VIRUS
CA of PML (Progressive Multifocal Leukoencephalopathy)
JC VIRUS (polyomaviridae)
CA or SPONGIFORM ENCEPHALOPATHY
PRIONS
Transport of spx for VIRUS:
4’C (ref); if delay of 3days
Transport of virus spx and sputum
4 days delay: -70’C
Used to hold cell culture tube.
Roller drum
Test for the presence of bacteria in milk
Methylene Blue reduction test
When culturing Cryptococcus in SDA, ____ Should NOT be in the medium.
CYCLOHEXIMIDE
Geotrichum candidum colonies appear as:
Rapid, yeastlike growth
Not a routine test in MICRO.
PCR
What does acridine orange stain
NUCLEIC ACIDS OF BACTERIA
Recovery of FUNGI:
Biopsy
CSF
ASPIRATIONS
(Never use swab)
C.diptheriae in Potassium tellurite (color)
Gray black
▪️Common component of the normal floral found on human skin and mucous membrane.
▪️disregarded by physician as a contaminant when found in blood culture.
▪️opp. Pathogen causing human disease: ENDOCARDITIS and SEPSIS
▪️pleomorphic, gram pos rods with single cells, V forms, or Chinese letters
Corynebacterium amycolatum
Preferred concentration technique for parasite ova and cyst
Formalin-ethyl-acetate sedimentation
Staph RESISTANT to 5ug novobiocin
S.saprophyticus
Staph SENSITIVE to 5ug novobiocin
S.epidermidis
Positive rxn for Beta lactamase chromogenic cephalosporin method
Color change
DNAse + bacteria (3)
SMS
S.aureus
Moraxella
Serratia
How many units of bacitracin (Taxo A) is used for the antibiotic susceptibility testing of group A B-hemolytic strep.
0.02-0.04
Used for the antibiotic susceptibility testing of group A B-hemolytic strep.
Bacitracin (taxo A)
How much optochin (taxo p) is used for the AST of S.pneumoniae.
5 ug
Used for the AST of S.pneumoniae
Optochin (5ug)
Result of genus Enterococcus in Bile Esculin test.
Positive
Positive result for Quellung test
Capsular swelling due to an antigen-antibody rxn
Used to differentiate Serratia from Klebsiella
- Failure to prod gas fr. Inositol
- Slowness and reluctance to ferment lactose
- Rapid gelatin liquefaction
This spp supplies V factor (NAD) for Haemophilus
Staphylococcus
String test is used for the dx of:
Vibrio cholarae
Coynebacterium spp that has the same morphology as C.diptheriae on BAP
C.ulcerans
Tests used to differentiate M.tuberculosis from M.bovis
Niacin test
Nitrate reduction test
M.tuberculosis: +
M.bovis: -
Dse caused by pulmonary form of anthrax
WOOLSORTER’S dse
Caused by CUTANEOUS form of anthrax
BLACK-ESCHAR
In water bacteriology, the following are used as CONFIRMATORY TEST MEDIA:
- ENDO AGAR
- EOSIN-METHYLENE BLUE AGAR
- BRILLIANT GREEN LACTOSE BROTH
Taxonomic naming of microorganisms:
▪️Uses binomial nomenclature in Id organisms
▪️Genus name can be abbreviated using the 1st letter
▪️uses greek names or words
Preferred incubation temperature (in degree Celsius) for bacteriologic culture
35+/-2
Most pathogenic bacteria that grows best in what environment
MOIST
Most common pathogenic isolate from the throat
Streptococcus pyogenes
Catalase and PYR hydrolysis negative
Bacitracin: R
CAMP: +
Streptococcus agalactiae
Most common agent of nosocomial UTI
S.epidermidis
Oxidase positive organisms
PPAM ViC HeN
Pseudomonas
Plesiomonas
Aeromonas
Moraxella
Vibrio
Campylobacter
Helicobacter
Nesseria
Flat with irregular, serrated edges and swirling phenomenon (MEDUSA HEAD COLONIES) in culture media
Bacillus anthracis
Generally, virologists categorize viruses according to the ____, then accdg to ______
Type of genome
Virus families
Used for the dx of C.albicans
Serum tube test (Germ tube test)
A method used for detecting oocysts
Schaudinn’s method
Parasite that exhbits DOUBLE-WALLED CYSTS with an OUTER WRINKLED WALL and an INNER POLYGONAL WALL.
Acanthamoeba castellani
Beef tapeworm
T.saginatta
Pork tapeworm
T.solium
CA of:
▪️Visceral Leishmaniasis (Kala-azar/Dum-dum fever)
Leishmania donovani
CA of CUTANEOUS LEISHMANIASIS ▪️cutaneous ulcer ▪️oriental sore ▪️classical self-limited ulcer ▪️Jericho boil ▪️Aleppo boil ▪️Delhi boil
Leishmania tropica
CA of MUCOCUTANEOUS Leishmaniasis
▪️ESPUNDIA
▪️UTA
Leishmania braziliensis
Gold std method, as well as DOH recommended method to DETECT and ID MALARIAL SPP
Thick and thin smear
▪️Normal flora of the upper RT
▪️Medium to large smooth and translucent colonies kn CHOCOLATE AGAR
▪️NON-hemolytic on BAP
▪️requires V factor
H.parainfluenzae
Growth:
➕: V factor
➖: X factor
* LIGHT GROWTH AROUND: XV Disk
V factor requirement
H.para-
Intracellular replicative form of the Chlamydia spp.
RETICULATE BODY
▪️An enhanced arrowhead hemolysis at the juncture of a culture
▪️Streak of beta-lysin-producing strain of S.aureus on SBAP
MEANS?
The beta-lysin of S.aureus acted SYNERGISTICALLY with the culture enhancing the hemolysis
In the HARADA MORI technique:
▪️float (top):
▪️settle (bottom):
▪️float (top):
STRONGYLOIDES filariform larva
▪️settle (bottom):
Hookworm filariform larva
Important cause of
CHORIORETINITIS:
TOXOPLASMA
Trichuris trichura infxn provides a good site for what parasite?
Entamoeba histolytica
Most common fungal contaminant of COMMERCIALLY prepared food
Rhizopus
