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MICROBIOLOGY (CUA) > RECALLS MICRO > Flashcards

RECALLS MICRO Flashcards

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1
Q

Part of the virus in which the envelope is required.

A

Nuclear/Cytoplasmic membrane

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2
Q

Eosinophilic cytoplasmic inclusions are elementary bodies of ___virus.

A

Poxvirus

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3
Q

Incubation period for fungi that grow on blood.

A

28 days

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4
Q

Parasites that have neither a cyst form. (2)

A

Dientamoeba fragilis

Trichomonas vaginalis

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5
Q

Roundworm that inhabits small intestine and usually demonstrated as rhabditoform larva.

A

Strongyloides stercoralis

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6
Q

Calcium alginate swab is used for spx taken fr. nasopharynx for the isolation of ______.

A

Corynebacterium diphtheriae

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7
Q

Used to isolate S.aureus from stool cultures.

A

Medium with 7.5%salt.

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8
Q

These are used as counterstain for acid fast staining.

A

Malachite green

Methylene blue

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9
Q

2 examples of anaerobic bacteria.

A

Veilonella

Actinomyces

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10
Q

+ result for bile solubility test

A

Lysis of the semisolid media

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11
Q

This bacteria does not contain a capsule.

A

N.meningitidis

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12
Q

Bacteria that produces Babes-Ernst granules.

A

C.diptheriae

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13
Q

How to prepare an agar medium?

A

Half water ➡️ Agar ➡️ Half water

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14
Q

Composition of IODOPHORE

A

Iodine + Detergent

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15
Q

Composition of TINCTURE OF IODINE

A

Iodine + alcohol

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16
Q

Type of plasma used for Tube Coagulase test

A

RABBIT OR HUMAN PLASMA

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17
Q

Lecithinase (+)

BULL’S EYE HEMOLYSIS

A

C.perfringens

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18
Q

Lowest concentration of drugs that inhibits bacterial growth.

A

MIC

Min. Inhibitory Concentration

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19
Q

Lowest concentration of drugs that kills/destroys organisms.

A

MLC

Min. Lethal Concentration

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20
Q

Gram neg, COFFEE-BEAN shaped diplococci with adjacent sides flattened

A

NEISSERIA spp.

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21
Q

Appropriate spx’s for culturing N.gonorrhea: (5)

A 
(GREVO)
Gastric fluid
Rectum
Eyes
Vaginal discharge 
Oral Cavity
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22
Q

Grows on CYSTEINE TELLURITE AGAR

A

C.diptheriae

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23
Q

CA of WHOOPING COUGH

A

Bordatella pertussis

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24
Q

It inhibits growth of gram pos and fungi in MAC agar.

A

BILE Salt

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25
Q

C.albicans mouth infection

A

THRUSH

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26
Q

Parasite that possess rhabditiform larva in stool.

A

S.stercoralis

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27
Q

Types of Plasmodium which accounts for 90% of cases (2)

A

P.vivax

P.falciparum

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28
Q

Example of an ECTOPARASITE

A

CRABS 🦀

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29
Q

Most predominant form of Plasmodium causing MALARIA IN THE WORLD

A

Vivax

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30
Q

Most predominant form of Plasmodium causing MALARIA IN THE PHILIPPINES

A

Falciparum

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31
Q

Agent of GAY BOWEL SYNDROME

A

Giardia lamblia

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32
Q

3rd Taenia spp

A

Asiatica

*common name: Taiwan Taenia

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33
Q

Taenia spp having a uterus that is DICHOTOMOUS / TREE-LIKE BRANCHES 🌲

A

T.saginata

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34
Q

Taenia spp having a uterus that is DENDRITIC OR FINGER-LIKE 🤚

A

T.solium

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35
Q

The adult resembles the adult of SPIROMETRA

A

D.latum

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36
Q

Resembles the egg of P.westermani

A

D.latum

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37
Q

+ ctrl for ACETATE TEST

A

E.coli

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38
Q

Acetate test:
Incubation period
+ result

A

35’C for 7 days

BLUE

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39
Q

ACETAMIDE TEST
+ctrl
INCUBATION PERIOD
+result

A

Pseudomonas aeruginosa
35’C for 7 days
BLUE

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40
Q 
MALONATE TEST
\+ CRTL
INCUBATION PERIOD
\+ result
- result
A

Salmonella
35’C for 7 days
BLUE +
GREEN/YELLOW -

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41
Q

Used in KATO-THICK Smear preparation

A

Cellophane paper soaked in mixture of GLYCERINE + MALACHITE GREEN/METHYLENE BLUE SOL’N

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42
Q

For detection of stipplings, how long will you need to prepare blood films after the blood is drawn?

A

30 MINS to 1Hr

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43
Q

Site of the preferred blood spx for blood smear for PROTOZOA

A

Finger puncture

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44
Q

Definitive host to Plasmodium

A

Female anopheles mosquito

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45
Q

Color of CHROMATID BODIES WITH TRICHROME STAIN

A

BRIGHT RED

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46
Q

Appearance of glycogen in the trophozoite after staining with trichrome stain.

Nucleus?

A

COLORLESS : glycogen

RED-PURPLE : Nucleus

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47
Q

Appearance of Charcot Leyden crystals in the trophozoite after staining with trichrome stain.

A

BRIGHT RED

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48
Q

Chromatoidal bodies of protozoan cysts in iodine

A

DO NOT STAIN

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49
Q

Stains used for: NAEGLERIA and ACANTHAMOEBA

A

H&E

WRIGHT’s stain

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50
Q

Destroyed by iodine

A

Trophozoites

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51
Q

Hexacanth embryo:
(Hymenolepis spp)
▪️polar filaments (+)
▪️polar filaments (-) lacks

A

H.nana (+)

H.diminuta (-)

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52
Q

The egg of ___ have hexacanth embryo in a radially striated shell

A

Taenia

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53
Q

2nd IH of D.latum

A

FRESH WATER CRABS

CRAYFISHES

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54
Q

Method for recovery of eggs of S.japonicum and S.mansoni

A

Rectal biopsy

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55
Q

These are HERMAPHRODITIC

A

Flukes

Tapeworms

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56
Q

Nematode parasites capable of HEART LUNG MIGRATION

A

ASH
A.lumbricoides
S.stercoralis
Hookworms

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57
Q

“Unholy three” or “Triad of Infection”

A

HAT
Hookworms
A.lumbricoides
T.trichura

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58
Q

Ag that is cross-reactive in ALL ADENOVIRUSES

A

Hexon

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59
Q

CA of Farmer’s Lungs

A

Aspergillus

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60
Q

Media used to ID C.albicans through the organisms’ CHLAMYDOSPORES production

A

CORNMEAL AGAR PLUS TWEEN 80

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61
Q

C.albicans produces (3)

A

BLASTOSPORES
CHLAMYDOSPORES
PSEUDOHYPHAE

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62
Q

Stain in AMAN STAIN

The most common fungal stain

A

LPCB

63
Q

Setting of RPMs marked on the face of the RHEOSTAT CTRL ON THE CENTRIFUGE. Checking?

A

Monthly

64
Q

Checking of:
CATALASE
OXIDASE
COAGULASE

A

Once each day of use

When vial is 1st used

65
Q

THIOGLYCOLLATE broth is boiled for ___ mins to?

A

10 mins

Drive off O2

66
Q

Clear medium for MYCOBACTERIA

A

Middlebrook 7H11

67
Q

Bordatella spp with is both UREASE AND OXIDASE +

A

B.bronchiseptica

68
Q

Preferred medium for the isolation of B.pertussis

A

CHARCOAL-CEPHALEXIN AGAR

69
Q

Inhibitors in SALMONELLA-SHIGELLA AGAR (2)

A

Bile salts

Brilliant green

70
Q

Methods for testing Beta lactamase (3)

A

Chromogenic-Cephalosporin Mtd
Acidometric mtd
Iodometric mtd

71
Q

Transcribe PPNG

A

Penicillinase-Producing Gonococci

72
Q

ZOI:

SENSITIVE FOR OPTOCHIN DISK TEST (Taxo P)

For Bacitracin Disk Test
(Taxo A)

A

> |= 14mm (taxo P)

Any ZOI = Sensitive

73
Q

Most common pathogen in throat cultures.

A

GROUP A Strep (S.pyogenes)

74
Q

Staph that is DNAse:
+
-

A

S.aureus

S.epidermidis

75
Q

Carrier used in COAGGLUTINATION by staph.

A

Staphylococcal PROTEIN A

76
Q

Can be used for Serotyping by swelling

A

Capsule

77
Q

Milk is considered as GRADE A if the bacterial ct is _____ per mL when RAW and not exceed ____ bacteria per mL once pasteurized

A

75K

15K

78
Q

Stain used to demonstrate spores

A

Schaeffer-Fulton

79
Q

Nasopharyngeal swab spx is recommended for detection of (3)

A

B.pertussis
N.meningitidis
H.influenzae

80
Q

Virus that can cause SHINGLES

A

VZV

81
Q

Culture media for Fungi (3)

A

SBA
POTATO DEXTROSE AGAR
CORNMEAL AGAR

82
Q

Broad spectrum antibiotics

A

Act against…

  1. gram pos
  2. gram neg
  3. Bacterial and non bacterial
83
Q

Number of nuclei of mature cyst of Entamoeba polecki

A

1

84
Q

Heat resistant fungi commonly associated as FOOD CONTAMINANT

A

Aspergillus

85
Q

Parasite that has characteristic AUTO-FLUORESCENCE

A

Cyclospora cayetanensis

86
Q

Organism having grayish white, transparent to translucent, matte or glossy colonies with a large ZONE OF BETA HEMOLYSIS

A

Group A (S.pyogenes)

87
Q

Types of blood for the detection of microfilaria are (2)

A

CAPILLARY

ARTERIAL

88
Q

Proglottid filled with eggs

A

Gravid

89
Q

Virus that can affect CNS

A

RABIES

90
Q

Most common mtd for toxoplasma detection

A

Detect specific ab

91
Q

Continent where Chagas Dse was 1st discovered

A

America

92
Q

Transcribe ToRCHeS

A 
Toxoplasma gondii
Rubella
CMV
HERPES
HEPATITIS
SYPHILIS
93
Q

This stains CELL WALL of fungi

A

Calcoflour white

94
Q

Obligate microaerophiles

A

Campylobacter

Helicobacter

95
Q

Coccidia that can be contacted from cats

A

Toxoplasma gondii

96
Q

Mechanical/phoretic vector or carrier of Ascaris eggs

A

Cockroaches

97
Q

Toxic to viruses
Toxic to Neisseria spp
Toxic to Chamydia spp

A

Calcium alginate swab - ✖️viruses

Cottons swab - ✖️ Neisseria

Wood - ✖️Chlamydia

98
Q

Chain of infection

A

Source
MOT
Susceptible Host

99
Q

Appropriate ratio of blood:broth for BLOOD CULTURE

A

1:5

100
Q

MOT OF HTLV I and II

A

Blood
Serum
Saliva
Semen

S3B

101
Q

Best types of water to prepare an agar medium are:

A

Deionized

Distilled

102
Q

Is rejected for the the dx of RSV

A

Stool

103
Q

Test used to determine C.tetani

A

Morphology

Tennis racket; lollipop; DRUMSTICK

104
Q

AFB FOUND IN NASAL MUCOSA

A

Not dx’c - NF

105
Q

Dx’C lab media (agar) for bacterial isolation has pH of

A

7.0 to 7.5

106
Q

Test used to diff. S pyogenes and S.pneumoniae from other strep.

A

Hemolysis test
Beta - S.pyogenes
Alpha - S.pneumoniae

107
Q

A tests used to diff.
Listeria
Corynebacteria

A

BILE ESCULIN HYDROLYSIS TEST
MOTILITY TEST

LISTERIA +
CORYNEBACTERIA -

108
Q

Filamentous bacteria which is the common source of making antibiotics

A

Streptomyces

109
Q

Too much moisture in disk diffusion in growth in AST leads to:

A

Smaller ZOI

110
Q

In SSA GIVE APPEARANCE OF:
Salmonella
Shigella

A

Salmonella:
▪️colorless colonies with Black centers ; H2S +

Shigella
▪️colorless colonies without Black centers ; H2S -

111
Q

Best culture media for Salmonella culture: (3)

A

SSA
XLD
TETRATHIONATE

112
Q

Sterilization in cans can kill:

A

endospores of:
▪️C.botulinum
▪️E.coli
And toxins

113
Q

Concentration of H2O2 as DISINFECTANT

A

3%

114
Q

Most ideal for GS/CS of ambulatory px

A

Random urine spx

115
Q

Most ideal for GS/CS for BEDRIDDEN PX

A

Catheterized urine

116
Q

Most ideal for GS/CS of WOMEN

A

Midstream clean-catch

117
Q

Alternative disinfectant if Na hypochlorite (chlorox) is unavailable in the lab.

A

Vinegar (acetic acid)

118
Q

Refers to the property to see the cellular details that are microscopically micrometers

A

Resolution

119
Q

Can be ruled out in SBA Plate

A

Haemophilus (fastidious org: HACEK)

120
Q 
Bacitracin - R
Hippurate hydrolysis test - neg
Bile esculin hydrolysis - pos
6.5% NaCl - pos
Beta hemolytic
A

Enterococcus

121
Q

2 purpose of SIM (Sulfide Indole Motility)medium

A

▪️motility

▪️tryptophan medium

122
Q

Soap is:

A

Germicidal

123
Q

Microsporum that fluoresce on WOOD’s UV LAMP

A

M.canis

124
Q

Agar for DERMATOPHYTES

A

Mycosel Agar

125
Q

This qualifies sputum for culture

A

GS

126
Q

Test that speciates Neisseria spp.

A

Sugar Utilization (CHO UTILIZATION TEST)

127
Q

Bacteria causes UTI (3)

A

E.coli
S.saprophyticus
Enterococci

128
Q

Complicate CYSTIC FIBROSIS PX (2)

A

P.aeruginosa

Burkholderia cepacia

129
Q

Causes SWIMMER’S Ear and burns

A

P.aeruginosa

130
Q

Acceptable spx for S.aureus

A

Nasal swab

131
Q

Niacin +
Nitrate reduction +
Catalase -

A

MTB

132
Q

Lecithinase +

Double zone of hemolysis

A

C.perfringens

133
Q

Four bacteria that are INHIBITED BY SPS DISK TEST:

A

G.vaginalis
Neisseria
Streptobacillus moniliformis
Peptostreptococcus anaerobius

134
Q

Phenylalanine Deaminase (PAD) +
Urease+
Lysine decarboxylase +

A

PPM
PROTEUS
PROVIDENCIA
MORGANELLA

135
Q

Test that has a positive result of RED: (5)

A 
INDOLE
MR
VP
UREASE
NITRATE
136
Q

Test that has a positive result of GREEN, neg color of BLUE: (4)

A

CAMA

Citrate
Acetamide
Malonate
Acetate utilization tests

137
Q

Test that has a positive result of PURPLE: (4)

A

L2OH

LIA
LOA (decarboxylase)
Oxidase
hippurate hydolysis

138
Q

Test that has a positive result of YELLOS: (3)

A

TOT

TSI

ONPG

TCBS (thiosulfate-citrate-bile salt-sucrose)

139
Q

Salmonella spp that is LYSINE & H2S neg

A

S.paratyphi A

140
Q

Test that diff Enterobacteriaciae

A

IMViC

141
Q

Doesn’t grow on MAC: (3)

A

MEK

Moraxella
Eikenella corrodens
Kingella kingae

142
Q

Pigment producer and causes grape-like odor on CETRIMIDE AGAR

A

P.aeruginosa

143
Q

Pigment produced by P.aeruginosa

A

Pyocyanin
Pyoverdin
Pyorubin

144
Q

Grows in CAP,
✖️MAC
✖️BAP

A

Haemophilus

145
Q

Bacteria known for its requirement cysteine which causes lab acquired infxn : BSL III

A

Francisella tularensis

146
Q

BSL I

A

My BaS

Mycobacterium gordonae
Bacillus subtilis

147
Q

BSL II

A

Y Ba?

Yersinia pestis
B.anthracis

148
Q

BSLIII

A

My FRiend BruMo

Mycobacteria
Francisella
Brucella
Molds

149
Q

Can speciate Brucella spp.

A

Thionine

Fuchsin

150
Q

Brucella spp
CO2 +
H2S +
Inhibited by THIONINE

A

B.abortus

151
Q

“Safety pin” appearance in Wayson’s stain (bipolar bodies)

▪️ acquired through animal bite wounds

A

Pasteurella

152
Q 
Causes:
▪️MENINGITIS
▪️SEPSIS
▪️STILLBIRTH
▪️FOOD POISONING
A

Listeria

153
Q

The ONLY GRAM POS BACILLUS
H2S +
Catalase -
“BUTCHER’s CUT”

A

Erysipelothrix rhusiopathiae

154
Q

CA of PONTIAC FEVER (fr. Aircondition)

CM: BCYE

A

Legionella

MICROBIOLOGY (CUA) (2 decks)

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