recall test Flashcards
explain how the structure of starch relates to its function?
-starch has a main role of energy storage
-insoluble in water, therefore isnt affected by osmosis or affects water potential
-compact meaning lots can be stored
-large therefore doesn’t diffuse out of cells
describe the test for reducing sugars
-add benedict’s reagent to a reducing sugar (glucose)
-heat in water bath for 5 minutes
-a positive result would end in a brick red colour, suggesting a large amount of reducing sugar
-the colour change can also vary from orange, yellow, green dependant on amount of reducing sugar
-a negative result would remain blue
what kind of bonds would you find between glucose?
-when glucose molecules link together in a long chain, polysaccharides are formed
-the link formed between each molecule is known as a glycosidic bond
-these glycosidic bonds are formed by condensation reactions
what kind of bonds would you find between glycerol and fatty acids?
-triglycerides contain three ester bonds between the fatty acids and the glycerol molecule formed by condensation reactions
-an ester bond forms when a hydroxyl group from the glycerol (OH) and carboxyl group from the fatty acid join (COOH)
-the H from the glycerol combines with the OH from the fatty acid to make water
what kind of bonds would you find between amino acids?
-each amino acid is attached to another amino acid by a covalent bond, known as a peptide bond
-in order to from a peptide bond a hydroxyl group (OH) is lost from the carboxyl group of one amino acid and a hydrogen atom is lost from an amine group of another amino acid
explain why cells need to be placed in a cold, buffered solution that is the same water potential as the tissue before cell fractionation
-the solution needs to be cold to reduce activity of enzymes that break down organelles
-it needs to be buffered to maintain a constant pH as could affect organelle structure
-it also needs to have the same water potential to prevent water from moving into the organelles via osmosis, causing them to expand/burst
describe the function of ribosomes
-produce proteins by translating genetic information from DNA
describe the function of the rough endoplasmic recticulum
-synthesises proteins
describe the function of mitochondria
-produce energy through cellular respiration
describe the events of prophase
-chromosomes condense becoming shorter and thicker
-nucleolus and nuclear envelope start to break down
-centrioles move to the opposite poles of the cell
-spindle fibres start to develop
name the 3 stages of the cell cycle
-interphase
-nuclear division
-cytokinesis