Reagents and Methods

Question 1

Which procedure tests commercial anti-A and anti-B against unknown patient or donor red blood cells?

Answer 1

ABO forward group

Question 2

Which procedure uses known commercial red blood cells (A and B antigens) to test unknown patient or donor plasma?

Answer 2

ABO reverse group

Question 3

Which procedure tests known commercial screen cells against unknown patient or donor plasma?

Answer 3

Antibody screen/antibody identification

Question 4

ABO type of antibody screening cells

Answer 4

Type O

Question 5

Coombs Control Cells

Answer 5

Also known as check cells
Used as the confirmatory step in the AHG phase of testing
Made from group O cells coated with human globulin

Question 6

Red cell antibodies enhanced by enzymes

Answer 6

Rh
Lewis
Kidd (example Jka and Jkb)

Question 7

Red cell antibodies destroyed by enzymes

Answer 7

Duffy (example FYa and Fyb)
M,N,S

Question 8

Enhancement agent that affects the second stage of agglutination

Answer 8

Bovine serum albumin (BSA)

Question 9

Enhancement agent that increases the rate of antibody uptake during the first stage of agglutination

Answer 9

Low ionic Strength Solution (LISS)

Question 10

Enhancement agent that works by concentrating antibody in a low-ionic strength solution

Answer 10

Polyethylene Glycol (PeG)

Question 11

Enhancement agent that reduces negative charges from the surface of the red cell during the first stage of agglutination

Answer 11

Enzymes (example ficin and papain)

Question 12

Test that detects presence of red blood cells sensitized in vivo (in the body)

Answer 12

Direct Antiglobulin Test(DAT)