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Aubf Lab Finals (2024) > Reagent Strip Parameter > Flashcards

Reagent Strip Parameter Flashcards

1
Q

Specific gravity reagent

A

Bromthymol blue

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2
Q

Specific gravity principle

A

Pka change of poly electrolytes

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3
Q

pH reagent

A

Methyl red and bromthymol blue

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4
Q

pH principle

A

Double indicator

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5
Q

Protein reagent

A

Tetrabromthymol blue

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6
Q

Protein principle

A

Protein error indicator

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7
Q

Glucose reagent

A

Glucose oxidase and peroxidase

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8
Q

Glucose principle

A

Double sequential enzyme

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9
Q

Blood reagent

A

Tetramethyl benzidine

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10
Q

Blood principle

A

Pseudoperoxidase activity of hemoglobin

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11
Q

Ketones reagent

A

Sodium nitroprusside

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12
Q

Ketones principle

A

Sodium nitroprusside reaction

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13
Q

Bilirubin reagent

A

Dichloroaniline diazonium salt

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14
Q

Bilirubin principle

A

Diazo reaction

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15
Q

Urobilinogen reagent

A

P-dimethylaminobenzaldehyde

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16
Q

Urobilinogen principle

A

Ehrlich’s reaction

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17
Q

Nitrite principle

A

Greiss reaction

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18
Q

Nitrite correspond

A

Nitrite correspond 100,000 CFU/mL

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19
Q

Leukocyte reagent

A

Leukocyte esterase

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20
Q

Leukocyte principle

A

Diazo reaction

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21
Q

Label in urine container

A

Name
Age/gender
Record time received

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22
Q

ml of urine needed

A

10-15ml

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23
Q

Errors of urine sample

A

Urine is insufficient
Sample insufficient

24
Q

Determine transparency

A

Clear
Hazy
Cloudy
Turbid
Milky

25
Q

Parameters of reagent strip

A

Specific gravity
pH
Protein
Glucose
Blood
Ketones
Bilirubin
Urobilinogen
Nitrite
Leukocyte

26
Q

Preliminary information physical examination

A

Glomerular bleeding
Inborn error of metabolites
Urinary tract infection
Liver disease

27
Q

Time for glucose

A

30 sec

28
Q

Time for bilirubin

A

30 sec

29
Q

Time for ketones

A

40 sec

30
Q

Time for SG

A

45 sec

31
Q

Time for pH

A

60 sec

32
Q

Time for protein

A

60 sec

33
Q

Time for blood

A

60 sec

34
Q

Time for urobilinogen

A

60 sec

35
Q

Time for nitrite

A

60 sec

36
Q

Time for leukocytes

A

120 sec

37
Q

Presence of casts, increase amount of ________,__________ or ____________indicate urinary tract infection and/or a renal disease.

A

Pus cell, blood cell or bacteria

38
Q

Presence of casts, increase amount of pus
cells, blood cells or bacteria indicate ___________ and/or a _______________.

A

urinary tract infection and/or a renal disease

39
Q

Centrifuge urine sample of 10-15ml in a tube for about

A

5 mins

40
Q

Centrifuge urine sample of 10-15ml in a tube for about 5mins of ________ RCF

A

400 RCF (relative centrifugal force)

41
Q

are identified and counted under a high power objective in at least 10 high power fields. Report as cell/hpf.

A

Red blood cells, pus cells

42
Q

Examine under low power objective in at least 10 low power fields sediments with low refractive index like

A

Mucus thread
Urates
Bacteria
Epithelial cells
Crystal
Cast

43
Q

Report low power

A

Many
Few
Moderate

44
Q

Report high power

A

Cell/hpf

45
Q

presence of large amount of crystals, bacteria, yeast or any microorganism Perform in

A

Confirmatory testing

46
Q

Bilirubin : neg (b1)
Urobilinogen: +++

A

Hemolytic

47
Q

Bilirubin: - or + (b1 & b2)
Urobilinogen: ++

A

Hepatic

48
Q

Bilirubin: +++ (b2)
Urobilinogen: -/dec N

A

Obstructive

49
Q

Glucose : renal threshold

A

160-180 mg/dl

50
Q

Presence of intact rbc

A

Hematuria

51
Q

Intravascular hemolysis

A

Hemoglobinuria

52
Q

Rhabdomyolysis (increase muscle destruction)

A

Myoglobinuria

53
Q

Cloudy red or smoky red

A

Hematuria

54
Q

Clear red

A

Hemoglobinuria and myoglobinuria

55
Q

Urobilinogen specimen urine

A

Afternoon urine (2-4 pm)

Aubf Lab Finals (2024) (4 decks)

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