Reagent Strip Parameter Flashcards
Specific gravity reagent
Bromthymol blue
Specific gravity principle
Pka change of poly electrolytes
pH reagent
Methyl red and bromthymol blue
pH principle
Double indicator
Protein reagent
Tetrabromthymol blue
Protein principle
Protein error indicator
Glucose reagent
Glucose oxidase and peroxidase
Glucose principle
Double sequential enzyme
Blood reagent
Tetramethyl benzidine
Blood principle
Pseudoperoxidase activity of hemoglobin
Ketones reagent
Sodium nitroprusside
Ketones principle
Sodium nitroprusside reaction
Bilirubin reagent
Dichloroaniline diazonium salt
Bilirubin principle
Diazo reaction
Urobilinogen reagent
P-dimethylaminobenzaldehyde
Urobilinogen principle
Ehrlich’s reaction
Nitrite principle
Greiss reaction
Nitrite correspond
Nitrite correspond 100,000 CFU/mL
Leukocyte reagent
Leukocyte esterase
Leukocyte principle
Diazo reaction
Label in urine container
Name
Age/gender
Record time received
ml of urine needed
10-15ml
Errors of urine sample
Urine is insufficient
Sample insufficient
Determine transparency
Clear
Hazy
Cloudy
Turbid
Milky
Parameters of reagent strip
Specific gravity
pH
Protein
Glucose
Blood
Ketones
Bilirubin
Urobilinogen
Nitrite
Leukocyte
Preliminary information physical examination
Glomerular bleeding
Inborn error of metabolites
Urinary tract infection
Liver disease
Time for glucose
30 sec
Time for bilirubin
30 sec
Time for ketones
40 sec
Time for SG
45 sec
Time for pH
60 sec
Time for protein
60 sec
Time for blood
60 sec
Time for urobilinogen
60 sec
Time for nitrite
60 sec
Time for leukocytes
120 sec
Presence of casts, increase amount of ________,__________ or ____________indicate urinary tract infection and/or a renal disease.
Pus cell, blood cell or bacteria
Presence of casts, increase amount of pus
cells, blood cells or bacteria indicate ___________ and/or a _______________.
urinary tract infection and/or a renal disease
Centrifuge urine sample of 10-15ml in a tube for about
5 mins
Centrifuge urine sample of 10-15ml in a tube for about 5mins of ________ RCF
400 RCF (relative centrifugal force)
are identified and counted under a high power objective in at least 10 high power fields. Report as cell/hpf.
Red blood cells, pus cells
Examine under low power objective in at least 10 low power fields sediments with low refractive index like
Mucus thread
Urates
Bacteria
Epithelial cells
Crystal
Cast
Report low power
Many
Few
Moderate
Report high power
Cell/hpf
presence of large amount of crystals, bacteria, yeast or any microorganism Perform in
Confirmatory testing
Bilirubin : neg (b1)
Urobilinogen: +++
Hemolytic
Bilirubin: - or + (b1 & b2)
Urobilinogen: ++
Hepatic
Bilirubin: +++ (b2)
Urobilinogen: -/dec N
Obstructive
Glucose : renal threshold
160-180 mg/dl
Presence of intact rbc
Hematuria
Intravascular hemolysis
Hemoglobinuria
Rhabdomyolysis (increase muscle destruction)
Myoglobinuria
Cloudy red or smoky red
Hematuria
Clear red
Hemoglobinuria and myoglobinuria
Urobilinogen specimen urine
Afternoon urine (2-4 pm)