Reagent Strip (Chemical Examination of Urine) Flashcards

1
Q

Enumerate the 10 reagent strip parameter

A

Specific gravity
pH
Protein
Glucose
Ketones
Blood
Bilirubin
Urobilinogen
Nitrite
Leukocyte esterase

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2
Q

Principle of specific gravity?

A

pKa change of polyelectrolyte

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3
Q

Principle of pH?

A

Double indicator system

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4
Q

Principle of protein?

A

Protein error of indicator

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5
Q

Principle of glucose?

A

Glucose oxidase reaction

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6
Q

Principle of ketones?

A

Sodium nitroprusside reaction

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7
Q

Principle of blood?

A

Pseudo-peroxidase activity of heme

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8
Q

Principle of bilirubin?

A

Diazo reaction

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9
Q

Principle of urobilinogen?

A

Ehrlich reaction

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10
Q

Principle of nitrite?

A

Greiss reaction

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11
Q

Principle of leukocyte esterase

A

Granulocytic esterase reaction

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12
Q

Reaction time of specific gravity?

A

45 s

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13
Q

Reaction time of pH?

A

60 s

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14
Q

Reaction time of protein?

A

60 s

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15
Q

Reaction time of glucose?

A

30 s

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16
Q

Reaction time of ketones?

A

40 s

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17
Q

Reaction time of blood?

A

60 s

18
Q

Reaction time of bilirubin?

A

30 s

19
Q

Reaction time of urobilinogen?

A

60 s

20
Q

Reaction time of nitrite?

A

60 s

21
Q

Reaction time of leukocyte esterase?

A

120 s

22
Q

Reagent M of specific gravity?

A

Poly (methylvinyl ether) maleic anhydrite; Bromthymol blue

23
Q

Reagents of pH?

A

Methyl red; Bromthymol blue

24
Q

Reagent M of protein?

A

Tetrabromphenol blue

25
Q

Reagent M of glucose?

A

Glucose oxidase, peroxidase, KI

26
Q

Reagent M of ketones?

A

Sodium nitroprusside

27
Q

Reagent M of blood?

A

Diisopropylbenzene dihydroperoxide TMB

28
Q

Reagent M of bilirubin?

A

2,4-dichloroaniline diazonium salt

29
Q

Reagent M of urobilinogen?

A

paradiethylaminebenzaldehyde

30
Q

Reagent M of nitrite?

A

p-arsanilic acid, tetrahydrobenzoquinolinol

31
Q

Reagent M of leukocyte esterase?

A

Derivatized pyerole, amino acid ester, diazonium salt

32
Q

Reagent C of specific gravity?

A

Ethyleneglycol-bs (aminoethylether); Bromthymol blue

33
Q

Reagent C of protein?

A

Tetrachlorophenol-tetrabromosulfonphthalein

34
Q

Reagent C of glucose?

A

Glucose oxidase, peroxidase, TMB

35
Q

Reagent C of ketones?

A

Sodium nitroprusside and glycine

36
Q

Reagent C of blood?

A

2,5-dimethyl 2,5-dihydroperoxyhexane TMB

37
Q

Reagent C of bilirubin?

A

2,6-dichlorobeneze diazoniumtetrafluoroborate

38
Q

Reagent C of urobilinogen?

A

4-methoxybenzene diazonium tetrafluoroborate

39
Q

Reagent C of nitrite?

A

sulfanilamide, 3-hydroxy- 1,2,3,4-tetrahydro- ,8-benzoquinoline

40
Q

Reagent C of leukocyte esterase?

A

Indoxylcarbonic acid ester, diazonium salt