Reactions Chart

Question 1

Substrate: Starch
Enzyme (*?)
End products
Indication of + test
useful for which organism

Answer 1

enzyme - amylase and maltase (*)
end products - glucose
indication of + test - clearing, with iodine as reagent
organism - wide variety

Question 2

Substrate: Triglyceride
Enzyme (*?)
End products
Indication of + test
useful for which organism

Answer 2

Enzyme (?): lipase ()
End products: glycerol, fatty acids
Indication of + test: clearing
useful for which organism: wide variety

Question 3

Substrate: Casein
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 3

Enzyme (?): caseinase ()
End products: amino acids
Indication of + test: clearing
useful for which organism: wide variety

Question 4

Substrate: Gelatin
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 4

Enzyme (?): gelatinase ()
End products: amino acids
Indication of + test: liquid (+), solid (-)
useful for which organism: wide variety

Question 5

Substrate: Sucrose, glucose, lactose
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 5

Enzyme (*?): fermentation enzymes
End products: organic acids, alcohol, CO2, H2
Indication of + test: yellow broth and/or gas formed in durham tube
useful for which organism: facultatives

Question 6

Substrate: hydrogen peroxide
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 6

Enzyme (*?): catalase
End products: H2O, free O2
Indication of + test: bubbles formed
useful for which organism: to rule out (-) streptococcus

Question 7

Substrate: mannitol
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 7

Enzyme (*?): fermentation enzymes
End products: organic acids
Indication of + test: (+) mannitol fermentation showing a color change from red to yellow
useful for which organism: To identify staph aureus (suggests)

Question 8

Substrate: Fibrinogen (liquid)
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 8

Enzyme (?): coagulase ()
End products: fibrin (solid)
Indication of + test: solidification
useful for which organism: to identify staph aureus (definitive)

Question 9

Substrate: Hemoglobin and RBCs
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

• a disk sensitive

Answer 9

Enzyme (?): beta hemolysins ()
End products: amino acids and Iron
Indication of + test: complete clearing
useful for which organism: to identify strep pyogenes

• a disk sensitive

Question 10

Substrate: Hemoglobin and RBCs
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

• p disk sensitive

Answer 10

Enzyme (?): alpha hemolysins ()
End products: Biliverdin, etc.
Indication of + test: incomplete clearing or greening
useful for which organism: to identify strep pneumoniae

Question 11

Substrate: H2 and O2
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 11

Enzyme (*?): oxidase
End products: H2O
Indication of + test: color change from pink to purple to black within 60 seconds
useful for which organism: to identify neisseria

Question 12

Substrate: thiosulfate
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 12

Enzyme (*?): thiosulfate reductase
End products: sulfite and hydrogen sulfide gas
Indication of + test: H2S production by blackening (FeS) indicator
useful for which organism: Gr (-) rods

Question 13

Substrate: Cysteine
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 13

Enzyme (*?): Cysteine desulfurase
End products: pyruvic acid and hydrogen sulfide gas and ammonia
Indication of + test: H2S production by blackening (FeS) indicator
useful for which organism: Gr (-) rods

Question 14

Substrate: Tryptophan
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 14

Enzyme (*?): Tryptophanase
End products: indole, pyruvate, ammonia
Indication of + test: cherry red, indole (+), with Kovac’s reagent
useful for which organism: e. coli and enterobacter

Question 15

Substrate: glucose
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

• methyl red reagent used

Answer 15

Enzyme (*?): fermentation enzyme
End products: acids and CO2 and H2, pH of -4
Indication of + test: pH of -4, red changes, with methyl red reagent
useful for which organism: e. coli and enterobacter

Question 16

Substrate: Glucose
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

• V.P. reagent A and B used

Answer 16

Enzyme (*?): fermentation enzymes
End products: 2,3 Butanediol, H2O, and CO2
Indication of + test: brick red color, with V.P. reagents A and B used
useful for which organism: e. coli and enterobacter

Question 17

Substrate: Citrate
Enzyme (*?):
End products:
Indication of + test:
useful for which organism:

Answer 17

Enzyme (*?): citrase
End products: pyruvate and CO2 and excess sodium, makes alkaline pH
Indication of + test: growth medium is blue, with citrate indicator for pH
useful for which organism: e. coli and enterobacter