Post lab Questions

Question 1

exoenzymes and endoenzymes

Answer 1

exoenzymes act on substrates outside of the cell like macromolecules, where as endoenzymes act on smaller molecules like sugar inside the cell

Question 2

serological tests and examples

Answer 2

all tests involve specific antibody and antigen bonding, so if you have one of these then the other can be tested for. .
- precipitation: involve soluble antibodies and antigens that are visible in agar (ouchterlony plate)
- agglutination: involves cellular antigens binding to antibodies and forms a clump, blood typing is example
- ELISA reactions are color changing reactions where antibody or antigen is bound to a dye so color appears when bonded, examples is pregnancy test

Question 3

indirect presence of enzyme involves two things

Answer 3

the disappearance of substrate or appearance of end product. example for direct would be the cytochrome oxidase enzyme