RDNA

Question 1

Requirements of a vector DNA

Answer 1

Orisite
Restriction site
Promoter region 
Selectible marker site
Antibiotic resistance gene

Question 2

Types of nucleases/molecular scissors

Answer 2

Endonuclease

Exonuclease

Question 3

Restriction endonuclease from E.Coli

Answer 3

EcoRI

Question 4

Name one plasmid vector

Answer 4

pBR322

Question 5

Stabke form of DNA

Answer 5

cDNA

Question 6

Steps of cDNA formation

Answer 6

RNA isolation
Reverse transcriptase added
Primers added
DNA polymerase added
S1 nuclease added
cDNA complete

Question 7

Steps of rDNA technology

Answer 7

Isolation of DNA
Cutting of desired DNA strand by restriction endonuclease
Isolation of desired DNA strand
Cutting of plasmid DNA by restriction endonuclease
Insertion of desired DNA strand with plasmid DNA by DNA ligase
Incorporation of rDNA in host cell
Replication of rdna in host cell
Multiplication of host cell
Selection of host cell containing rDNA

Question 8

Steps of rDNA

Answer 8

Isolation
Cutting
Ligastion
Incorporation in host
Multiplication
Selection

Question 9

Antibiotic resistance in vector

Answer 9

Ampicillin resistance

Tetracycline resistance

Question 10

Central dogma

Answer 10

DNA replication
DNA transcription
RNA translation/RNA reverse transcription
Protein