RATs lecture quiz #1

Question 1

Mus musculus life span

Answer 1

1.5 - 3 yrs

Question 2

Mus musculus weaning age

Answer 2

19 - 21 days (same as gestation) eating solids and drinking on own

Question 3

Mus musculus lung lobes

Answer 3

R lung 4 lobes

L lung 1 lobe

Question 4

Mus musculus liver lobes

Answer 4

4 lobes

Median, Left, Right and Caudate

Question 5

Mus musculus mammary glands

Answer 5

F: 5 pair: 3 thoracic, 2 abdominal
M: 3 pair (no abdominal)

Question 6

“Bruce” effect

Answer 6

If a pregnant F comes in contact with the pheromones of a foreign M, she will abort

Question 7

“Lee-Boost” effect

Answer 7

suppression or prolongation of estrus cycle PSEUDO PREGNANT STATE

Question 8

“Whitten” effect

Answer 8

Sync’d estrous

Question 9

Vaginal plug

Answer 9

Can be seen within 18-24 hrs post-cop, usually gone by 48 hrs

Question 10

Altricial

Answer 10

Born blind, helpless

Opposite of “precocious”

Question 11

Mus musculus life cycle

Answer 11

Day 10 - grow hair
Day 12 -eyes open
Wk 2 - eating solids

Question 12

Mus musculus average size

Answer 12

20 - 25 gm

Question 13

Mus musculus body temp

Answer 13

97.5 F - 100.4 F

Question 14

Mus musculus daily food consumption

Answer 14

12 - 18gm per 100gm of body weight/day

Question 15

Mus musculus dental formula

Answer 15

2 (1/1; 0/0; 0/0; 3/3)

I, C, P, M

Question 16

Mus avg H2O consumption

Answer 16

15mL/100g b/w day

Question 17

Mus musculus estrous cycle

Answer 17

4-5 days

Question 18

Mus musculus ESTRUS cycle

Answer 18

10-12 hrs (period when animal can conceive)

Question 19

Mus gestation

Answer 19

19-21 days

Question 20

Mus reach puberty in

Answer 20

6 - 8 weeks

Question 21

Mus post postpartum ESTRUS

Answer 21

14 - 28 hrs (mouse can conceive again 14 hrs after giving birth!)

Question 22

Mus musculus avg litter size

Answer 22

6 - 12 pups

Question 23

Mus temp range

Answer 23

Ideal: 68F - 72F
Acceptable: 64F - 79F

Question 24

Mus humidity range

Answer 24

30% - 70%

Question 25

Mus light cycle

Answer 25

12 on/12 off

Question 26

Mus feed make up

Answer 26

16% protein/4%- 5% fat For breeders, increase protein

Question 27

I.D. methods for mice

Answer 27

Temporary: marker, hair dye, fur clipping, cage card
Permanent: ear notch, tag, microchipping, tattoo

Question 28

Acceptable euthanasia techniques for mice

Answer 28

2013 AVMA guide: decapitation, barbiturate o.d., cervical dislocation, CO2

Question 29

Types of lab facilities listed from dirtiest to cleanest

Answer 29

Conventional (no barrier)
Specific pathogen free  
-----------------------------------
Gnotobiotic  (known path free)
Axenic (free of ALL pathogens)

Question 30

Air changes per type of facility

Answer 30

Conventional: 1 per hr

HVAC 10 - 15 per hr

Question 31

What type of hood/bio safety cabinet?

Answer 31

LAMINAR flow hoods

Question 32

Define stock and strain as applied to Mus, as well as F1 hybrid, transgenic and knockout

Answer 32

Stock: outbred, genetically diverse
Strain: most genetically homologeous
Transgenic: replacing a gene in genetic makeup of animal
F1 hybrid: 1st gen of progeny from mating btw 2 DIFF known inbred strains
Knockout: removing a gene

Question 33

Genotype vs. Phenotype

Answer 33

Genotype: trait or gene
Phenotype: physical trait

Question 34

Purpose bred vs. random source

Answer 34

Purpose bred is Class A

Random source is USDA Class B - unknown genetic or medical background

Question 35

Research animal type breakdown

Answer 35

90% of all research animals are rats and mice
9% are dogs, cats, birds, etc
less than 1% are non-human primates
As of 2000, NO new chimps in research

Question 36

Research teams

Answer 36

P.I. = scientist, vet tech, husbandry, caretaker, vendors

Question 37

Three R’s of research

Answer 37

Reduce, Refine, Replace

Question 38

Important acronyms

Answer 38

IACUC: Institutional Animal Care and Use Comittee
NAVTA:Nat Asst of Vet Techs in Amer
ASLAP: Am Soc of Lab Animal Practitioners
NABR
FBR
ILAR: Institute of Lab An Research