Rate Of Enzyme Action

Question 1

Step one

Answer 1

Label 3 test tubes (one is pH, other is starch and then 3rd tube amylase)

Question 2

Step two

Answer 2

Place one drop of iodine solution into each depression on two spotting tiles.

Question 3

Step three

Answer 3

Add 2cm3 of starch solution to the test tube labelled starch. Add 2cm3 of amylase solution to the test tube labelled amylase. Add 2cm3 of your particular pH solution to the test tube labelled pH.

Question 4

Step four

Answer 4

Place all these labelled test tubes with solutions into the water bath (beaker of water from main water bath) and wait until all solutions have reached 30 °C

Question 5

Step five

Answer 5

Use the syringe to place 2cm3 of starch into the buffered pH solution test tube.

Question 6

Step six

Answer 6

Use another syringe to add 2cm3 of amylase to the starch + pH buffer testtube. Your pH test tube should now contain 2 cm3 starch, 2 cm3 amylase and 2cm3 pH.

Question 7

Step seven

Answer 7

Immediately start the timer and leave it on throughout the test.

Question 8

Step eight

Answer 8

Mix using a glass rod

Question 9

Step nine

Answer 9

After every 20 seconds, remove one drop of the mixture with a glass rod/pipette.

Question 10

Step ten

Answer 10

Place this drop on the first depression of the spotting tile with the iodine solution. The iodine should turn a blue-black colour.

Question 11

Step eleven

Answer 11

Rinse the rod

Question 12

Step twelve

Answer 12

Use the glass rod to remove one drop of the mixture every 20 seconds. Put each drop onto the iodine solution in the next depression on the spotting tile. Rinse the glass rod with water after each drop. Continue until the iodine solution and the amylase/buffer/starch mixture remain orange.

Question 13

Step thirteen

Answer 13

Repeat the procedure with solutions of other pHs

Question 14

Step fourteen

Answer 14

Record your results in a table