Rate Of An Enzyme Controlled Reaction

Question 1

What are the factors that affect enzyme activity?

Answer 1

Enzyme concentration, substrate concentration, temp, PH

Question 2

How is a control set up in a practical measuring enzyme activity?

Answer 2

Replace the enzyme solution with distilled water or boiled enzyme solution

Question 3

How can the results of the practical measuring enzyme activity be used to find the initial rate of reaction

Answer 3

Plot results on graph of rate of reaction against time draw a tangent at time = 0 to find the initial rate

Question 4

Practical procedure used to measure the effect of temp on enzyme activity using trypsin and milk

Answer 4

Immerse equal volumes of trypsin and milk, stored in different test tubes, in a water bath for 5 mins for temp to equilibrium. Mix together and immediately start timing record time take for milk to be hydrolysed test at-least 5 temps with at-least 3 repeats at each temp

Question 5

How is the rate of reaction calculated from time?

Answer 5

Rate of reaction =1/time

Question 6

What is the effect of temp on enzyme activity?

Answer 6

As temp increases kinetic energy increases so more ES complexes form rate of reaction increases up to the optimum temp.
Bonds in enzyme tertiary structure break changes shape of active site no longer complimentary so rate of reaction decreases

Question 7

What is the risk and level of risk associated with handling enzymes?

Answer 7

Allergic reaction avoid contact with skin wear eye protection