Calculating Mitotic Index Using Plant Cells

Question 1

Where in plants can cells undergoing mitosis be found?

Answer 1

Meristem tissue at shoot and root tips

Question 2

What is mitotic index

Answer 2

Ratio of cells undergoing mitosis to the total number of cells in a sample

Question 3

Outline the procedure to prepare a root tip slide

Answer 3

1. Warm 1m HCL to 60 degrees
2. Cut root tip with scalpel add to HCL leave for 5 mins
3. Remove and wash with distilled water
4. Cut the tip and place on slide add stain to make chromosomes visible

Question 4

Mitotic index

Answer 4

Number of cells with visible chromosomes/ number of cells in sample

Question 5

Hazards/ precautions of reagents used

Answer 5

HCL - corrosive avoid contact with skin wear eye protection
Toluidine - irritant avoid contact with skin wear eye protection
Scalpel - cut away from fingers