Rate Limiting Steps

Question 1

Fatty Acid Synthesis

Answer 1

Acetyl CoA Carboxylase
(Acetyl CoA -> Malonyl CoA)
- Need biotin as a cofactor

Question 2

Beta Oxidation

Answer 2

CPT I (2nd member of carnitate shuttle)
FA CoA --> FA CoA Carnitate

Question 3

Citric Acid Cycle

Answer 3

Isocitrate Dehydrogenase
Isocitrate –> a-ketoglutarate
1 NADH also produced

Question 4

Glycolysis

Answer 4

Phosphofructokinase-1
(Fructose-1-phosphate –> Fructose-1,6-biphosphate
1 ATP used

Question 5

Gluconeogenesis

Answer 5

Fructose-1,6-biphosphotase

Fructose-1,6-biphosphate –> Fructose-1,6-phosphate

Question 6

Beta Oxidation

Answer 6

CPT 1 (first unit of carnitine shuttle)