random stuff Flashcards
1
Q
what is western blotting used for
A
identification of specific proteins
2
Q
what are the steps in western blotting
A
prepare sample using detergent separate proteins on SDS-PAGE by size prepare transfer - membrane-gel sandwich between two sponge layers and add transfer buffer transfer of proteins - electrophoresis staining and visualisation
3
Q
role of detergent in western blotting
A
creates homogenate/lysate
breaks open plasma membrane so proteins can escape
4
Q
5 examples of tumour suppressors
A
DDC APC BRCA p53 Rb
5
Q
tumour suppressor that prevents progression from G1 to S phase
A
Rb gene (Retinoblastoma)
6
Q
normal function of APC
A
causes degradation of b-catenin complex by the ubiquitinin proteosome during wnt signalling
therefore prevents transcription
7
Q
2 hit hypothesis
A
both copies of tumour suppressor gene are required to be lost for loss of function
8
Q
normal function of Rb
A
prevents progression into S phase of cell cycle
causes transcription factor E2F to be inactive when bound to Rb
