Random Questions Flashcards
What are the virulence factors of Bacillus cereus
-Nonhemolytic enterotoxin
-Cytotoxin K
-Hemolysin BL
Virulence factors of Bacillus anthracis
- D-glutamate capsule
- Exotoxin with 3 components (edema factor, lethal factor & protective antigen)
A screening disk for Moraxella catarrhalis
Butyrate disk
What is the third common cause of otitis media
M. catarrhalis
Causes Ophthalmia neonatorum
Neisseria gonorrhoeae
Major virulence factor of N. meningitidis
capsule
Major virulence factor of N. gonorrhoeae
Pili
The typical morphology of N. gonorrhoeae
kidney or coffee bean-shaped
The reagent used in Modified Oxidase test
6% trimethyl-p-phenylene diamine dihydrochloride
Result of
Bacitracin: Lysostaphin and Furazolidone between Micrococcus spp and Staphylococci spp
Micrococcus is SENSITIVE to Bacitracin and RESISTANT to Lysostaphin and Furazolidone;
Staphylococci is RESISTANT to Bacitracin and SENSITIVE to Lysostaphin and Furazolidone
result of Neisseria spp in Oxidase test
Positive
The agar used in CHO utilization test in Neisseria
Cystine Trypticase Agar
True or False
Neisseria spp are cold-sensitive.
True
What is the other name for gonorrhea
clap disease
What is the color of a positive Oxidase test result for Neisseria
Purple
How to destroy anthrax bacillus?
autoclaving at 121 deg Celsius
Stain used in visualization of B. anthracis
Schaeffer and Fulton
Name the primary dye and counterstain in Schaeffer and Fulton
Primary dye: Malachite green (stains spores)
Counterstain: Safranin (stains vegetative cells)
Bamboo fishing rod on microscopy
Bacillus anthracis
A selective agar used for Bacillus anthracis
colonies appear creamy white, domed
PLET- Polymyxin Lysozyme EDTA Thallous Acetate
An agar used to detect lecithinase and lipase for Bacillus cereus
Egg Yolk Agar
Klebs Loefller Bacillus
Corynebacterium diphtheriae
Bull’s neck appearance
Corynebacterium diphtheriae
Transmission pattern in C. diphtheriae
Person-to-person exposure to respiratory droplets
Picket fence/pallisade/Chinese letters, x-y-v on microscope
C. diphtheriae
The metachromatic granules of C. diphtheriae is called
Babes Ernst
Gray to black colonies selective to c. diphtheriae
Cystine Tellurite Blood Agar
*potassium tellurite also produces gray to black colonies
This agar plate produces Black colonies with brown halo for C. diphtheriae
Tinsdale agar
Hemolytic pattern of c. diphtheriae on BAP
beta-hemolytic
It shows enhance granule and pleomorphism for C. diphtheriae, which agar is this?
Loeffler’s medium
What is the in vitro toxin test for Corynebacterium diphtheriae
Modified Elek’s test
What is the in vivo toxin detection test for Corynebacterium diphtheriae?
Animal inoculation/Guinea Pig Lethal Test
What is the positive result of Modified Elek’s test
Precipitin lines
An immunity test for diphtheria
Schick’s test
Lavender to lavender green on BAP
Stenotrophomonas maltophilia (maltose positive)
Purple on MAC
Acinetobacter
wrinkled colonies
P. stutzeri and B. mallei
A key pathogen in the lungs of cystic fibrosis patients
Pseudomonas aeruginosa
beta-hemolytic, taco/corn tortilla/overripe grapes odor on BAP
P. aeruginosa
The pigments produced by P. aeruginosa
pyocyanin (water-soluble blue) and pyoverdin (green)
Biochemical characteristics of P. aeruginosa
- Nitrate reduction positive
-LDC negative
-Positive grown on Cetrimide agar
-Positive growth in acetamide utilization
Gelatin hydrolysis test on other Pseudomonas
Positive = P. fluorescens
Negative = P. putida
Causes onion bulb rot
Burkholderia cepacia
Agent of Glandler’s disease
Burkholderia mallei
Earthy odor on Ashdown media
Burkholderia pseudomallei
Corn-flower blue colonies on EMb
Acinetobacter baumannii
Ammonia-like odor colonies
Acinetobacter baumanii
Characteristics of Acinetobacter baumanii on MAC and BAP
MAC: blue colonies w peach to pink tint
BAP: gummy colonies
Causes abortion, febrile disease
Brucella
Microscopy of Brucella
Gram negative
Coccobacilli w sand appearance
Media for isolation of Brucella
-Castañeda
-Trypticase Soy agar
-Tryptose agar
-Brucella broth agar w Wisconsin media (5% horse or rabbit serum)
Give the corresponding animal host of the ff.
Brucella abortus
Brucella melitensis
Brucella suis
Brucella canic
B. abortus = cows, camels
B. melitensis = sheep
B. suis = pigs
B. canis = dogs
Market men’s disease, deerfly fever
Tularemia
Susceptibility test for F. tularensis
Forshay test
Causes whooping cough
Bordetella pertussis
Causes septicemia and meningitis in men and kennel cough in dogs
Bordetella bronchiseptica
characteristics of Bordetella pertussis on Bordet-Gengou medium
Mercury droplets
Basic characteristics of Mycobacterium
Gram positive, Acid-fast, aerobic, and non-motile
Most recommended plating media for B. pertussis
Regan-Lowe Charcoal Agar
Composition of Regan-Lowe Chracoal agar
10% horse blood and cephalexin
Hansen’s disease
Leprosy
Has microscopiic characteristics of cigar packet arrangement
Mycobacterium leprae
What is the gold standard for digestion and decontamination
N-acetyl-L cysteine for digestion
NaOH for decontamination
What does the 3+ represent in Ziehl Neelsen-Kinyoun AF Staining?
More than 10 AFB/OIF in at least 20 VF
Result of Fluorochrome staining for Mycobacterium
AFB: Yellow against black bbackground
Which stains composes the Fluorochrome staining
Auramine rhodamine stain/TRUANT stain
What does 3+ represent in Fluorochrome staining reporting
4-36 AFB/field
What does 2+ represent in Fluorochrome reporting?
2-18 AFB/50 fields
What does 4+ represent in Flurochrome reporting?
> 36 AFB/field
What is the reporting if there is 1-2 AFB/ 70 fields in fluorochrome staining?
Doubting, Repeat using new specimen
In which tests is Lowenstein-Jensen Media being used for identifying Mycobacterium spp.?
In Niacin test and Iron Uptake test
It is also used as a selective and non–selective media
What reagent is used for the Iron Uptake Test of Mycobacterium?
Ferric Ammonium Citrate
What is the color reaction of Arylsulfatase test?
pink
