Plating Media Flashcards
For differentiation of non-fermentative gram-negative bacteria, particularly Pseudomonas aeruginosa
Acetamide agar
enrichment medium for the cultivation of Vibrio species from feces
Alkaline Peptone Water
Differential isolation medium and presumptive identification of group D streptococci and Enterococci
Bile Esculin Agar
Cultivation of non-fastidious microorganisms, determination of hemolytic reactions
Blood Agar
Isolation of Bordetella pertussis and B. parapertussis
Bordet-Gengou Agar
For cultiivation of fastidious organisms
Brain Heart Infusion Agar/broth
Enrichment of Legionella spp. Supports the growth of Francisella and Nocardia
Buffered Charcoal-Yeast Extract Agar (BCYE)
Highly selective medium used for isolation of Salmonella spp.
Bismuth Sulfide Agar
Selective holding medium for recovery of Campylobacter spp.
Campylobacter thioglycolate broth
Selective for Yersinia spp. maybe useful for isolation of Aeromonas spp.
Cefsulodin-Irgasan-Novobiocin (CIN) medium
For cultivation of fastidious organisms such as Haemophilus, Brucella spp, and Neisseria spp.
Chocolate Agar
Selective isolation of gram-positive cocci on heavily contaminated sample (stool).
Columbia Colistin Nalidixic acid agar
For isolation of Corynebacterium diphtheriae
Cystine-tellurite blood agar
Used for isolation and differentiation of Staphylococci based on mannitol fermentation and gelatinase activity
Chapman Stone medium
For cultivation of Francisella tularensis
Cystine Heart Agar
Used for cultivating Streptococci and Lactobacilli, particularly in dairy procedures
Elliker broth
Enrichment medium for Leptospira
Fletcher’s medium
Selective and differential medium for the isolation and differentiation of Salmonella and Shigella spp. from other gram-negative bacilli. [Bromthymol blue and acid fuschin as indicators]
Hektoen Enteric Agar (HEA)
Selective and differential medium for Gardnerella vaginalis
Human Blood Tween (HBT) bilayer
Isolation and growth of Corynebacterium spp.
Loeffler medium
For the isolation and differentiation of E. coli 0157:H7 in stool specimens.
MacConkey Sorbitol Agar
Selective differentiation of staphylococci
Mannitol salt agar
Recommended for the isolation of Lactobacillus spp.
MRS (deMan, Rogosa, and Sharpe)
Recommended for antimicrobial disk diffusion susceptibility teesting
Mueller Hinton Agar
Selective for Neisseria gonorrhoeae
New York City agar
Qualitative procedures for the isolation of Mycobacteria
Petragnani medium
Enrichment and selective medium for isolation of Bordetella pertussis
Regan Lowe
Selective for Campylobacter spp and Helicobacter spp
Schaedler agar
Enrichment of isolation of Salmonella spp.
Selenite agar
Selective for Campylobacter spp.
Skirrow agar and Campy-blood agar
Selective and differential for Vibrio spp.
Thiosulfate Citrate-Bile Salt Agar (TCBS)
Selective and enrichment for Streptococcus agalactiae in female genital specimens
Todd-hewitt broth
Isolation and differentiiation of Salmonella and Shigella spp. from other gram-negative enteric bacilli.[Phenol red as indicator]
Xylose Lysine Deoxycholate agar (XLD)