Rammage Tutorial Answers

Question 1

What is a good sample to take from a child

Answer 1

Simple salivary swab

Question 2

What organisms mostly detect for caries?

Answer 2

Step mutans

Lactobacillus species

Question 3

How would you isolate bacteria for caries?

Answer 3

NGS next generation sequencing

Question 4

How would one take a specimen when testing for periodontal bacteria?

Answer 4

Swab of subgingival plaque biofilm using a paper point

Question 5

What organism at cause for gingivitis?

Answer 5

Porphyromonas gingivalis

Question 6

How could one identify a culprit organism for gingival swab?

Answer 6

Selective agar

NGS

PCR

Question 7

How is cause / effect determined?

Answer 7

Remove culprit organism leads to resolution of disease

Question 8

What is main cause of denture biofilm?

Answer 8

Candida glabrata or Candida albicans

Question 9

How would one isolate denture biofilm species?

Answer 9

Oral rinse or swab from local area, foam pad or imprint too

Question 10

Any possible complications of not treating the Candida on the denture?

Answer 10

Possible aspiration pneumonia

Possible link to CV disease

Question 11

How might one take a specimen of an abscess? What bacteria would you look for

Answer 11

Needle aspiration

Oral anaerobes

Question 12

If you are looking for anaerobes what must you consider>

Answer 12

Transport and store in an anaerobic environment due to oxygen sensitivity