quizzes

Question 1

Spin columns use a silica based membrane to allow for DNA binding but only if the DNA solution contains appropriate components such as chaotropic reagents.

Answer 1

true

Question 2

After the plasmid DNA is bound to the spin column, the purpose of the wash steps that follow is to remove the bound plasmid DNA.

Answer 2

false

Question 3

If a restriction enzyme cuts only once (for example on the right hand side of the foreign DNA insert near the cloning site), then this will not release the DNA insert when examined afterwards by agarose gel electrophoresis.

Answer 3

true

Question 4

We expect that when we use restriction enzymes to cut the recombinant plasmids isolated from different colony picks from our transformation that this will generate fragments that are all the same size when analyzed by gel electrophoresis.

Answer 4

false, they will have different sizes

Question 5

After a gel electrophoresis run, smaller DNA fragments will be located closer to the top of the gel than larger DNA fragments because they migrate through the gel more slowly due to their smaller size.

Answer 5

false, they move more quickly

Question 6

Plasmid in which conformation will tend to migrate more quickly through an agarose gel:

Answer 6

Plasmid in a tight (e.g. supercoiled) conformation