Quiz Tomorrow

Question 1

These processes involved in genetic engineering and the applications of recombinant DNA technology.

Answer 1

Recombination DNA

Question 2

this process manipulates cells’ genetic information using laboratory techniques in order to change the traits of living organisms.

Answer 2

Genetic engineering

Question 3

an enzymatically controlled process where the plant or animal cells are treated with certain enzymes. Enzymes ) such as cellulase (plant cells), lysozyme (bacteria) and chitinase (fungi) are used to isolate pure DNA from the cells.

Answer 3

Isolation of DNA

Question 4

The isolated and purified DNA is treated with restriction endonucleases which cut the DNA into fragments. The restriction endonucleases are sequence-specific, typically palindrome sequences and snip the DNA at specific points.

Answer 4

Fragmentation of DNA

Question 5

a process to amplify the gene once the proper gene of interest has been cut using the restriction enzymes. Through this process, multiple copies of the gene of interest can be produced.

Answer 5

Polymerase chain reaction

Question 6

PCR proceeds in three stages,

Answer 6

denaturation, annealing and extension.