quiz 8

Question 1

cellular structures are visualized based on light emission by the specimen

Answer 1

fluorescence microscopy

Question 2

Fluorescence is a phenomenon where

Answer 2

chemical structure of a fluorophore
temporarily captures electromagnetic radiation of one wavelength (the excitation wavelength) and releases it as radiation of another, lower energy, wavelength (the emission wavelength.

Question 3

fluorescein isothiocyanate (FITC)

Answer 3

fluorescent dye

Question 4

To produce a ________ image, a specimen is illuminated with light at a wavelength that excites natural fluorescent pigments or synthetic fluorescent dyes that have been used to stain the material.

Answer 4

fluorescence

Question 5

When excitation light is directed onto the specimen from the viewing side, the method is known as ____________

Answer 5

epifluorescence.

Question 6

To keep reflected excitation light from
entering the optical system, a _______ that allows emitted wavelengths but not excitation wavelengths to pass is placed in the light path

Answer 6

dichroic mirror

Question 7

Sensitivity is enhanced by using _________which only allow emitted light of the desired wavelength range to pass through

Answer 7

emission filters