Quiz 5 review Flashcards
fMet-tRNAf enters the _______ of the _______ ribosome.
P-site, prokaryotic
EF-Tu and EF-G can both bind the A-site at the same time.
False
A(an) ___________ provides additional sequence context for directing the 30S ribosomal subunit to the site of translational initiation.
Shine-Dalgarno sequence
Approximately what percentage of cytoplasmic RNA is mRNA in a vertebrate cell?
approx. 5%
Ribosomes are fully assembled (or complete) only when in engaged in translation.
True
Translational termination is regulated by by specialized terminator tRNAs that recognize a stop codon.
False
“PAM” is an acronym for, _______________________________________ .
Protospacer adjacent motif
Which eukaryotic elongation factor is responsible for delivering an aminoacyl-tRNA to the A-site?
eEF1 alpha
The small ribosomal subunit is comprised of protein and RNA, with RNA making up more than half of the complex’s mass.
True
The PABP is essentially a eukaryotic initiation factor.
True
“CRISPR” is an acronym for, _____________________________________ .
Clustered regularly interspaced short palindromic repeats
The E-site in an 80S ribosome is necessary for releasing deacylated-tRNAs
False
“TALEN” is an acronym for _________________________________________ .
Transcription activator-like effector nucleases
Which statements represent technical barriers for using programmable nucleases to genome edit?
delivery to individual cells, delivery to a whole organism, off-target effects, poorly known target genome, and promiscuity
Aside from the initiator tRNA, aminoacyl-tRNAs enter the ribosome at the _________ site.
A
